This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Post-translational: Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
宿主种属
兔(Rabbit)
特异性
PCNA
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human PCNA (AA 100-160).
阳性对照
WB: HeLa, HepG2, U937, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human colon tissue. IF/ICC: HeLa cells. IP: HeLa cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant PCNA Antibody (Ab120680) - Western Blot All lanes: Recombinant PCNA Antibody (Ab120680) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 32 kDa Exposure time: 53.3 s
Recombinant PCNA Antibody (Ab120680) - IP Immunoprecipitate (IP) analysis using Recombinant PCNA Antibody (Ab120680) at 1/50 dilution. (WB with Recombinant PCNA Antibody (Ab120680) at 1/3000 dilution)
Recombinant PCNA Antibody (Ab120680) - IHC Immunohistochemical analysis of paraffin-embedded human colon, using Recombinant PCNA Antibody (Ab120680).
Recombinant PCNA Antibody (Ab120680) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant PCNA Antibody (Ab120680).
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