ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human PCNA Antibody, Recombinant (RR704), could be used for WB, Flow, IF/ICC, IP and so on.
Application:
WB: 1/1000 - 1/2000
Flow: 1/800 - 1/2000
IF/ICC: 1/1000
IP: 1/50
Recombinant PCNA Antibody (Ab120681) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 14.1 s
Recombinant PCNA Antibody (Ab120681) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant PCNA Antibody (Ab120681) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant PCNA Antibody (Ab120681) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant PCNA Antibody (Ab120681) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant PCNA Antibody (Ab120681) (1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant PCNA Antibody (Ab120681) - IP
PCNA was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant PCNA Antibody (Ab120681) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant PCNA Antibody (Ab120681) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant PCNA Antibody (Ab120681) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).
1/800 - 1/2000
1/50
1/1000 - 1/2000
1/1000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1011773 |
分析证书 | Ab120681 |
| ZJ25F0117218 |
分析证书 | Ab120681 |
| ZJ25F0117219 |
分析证书 | Ab120681 |
| ZJ23F0901112 |
分析证书 | Ab120681 |
| ZJ23F0901113 |
分析证书 | Ab120681 |
| ZJ26F0636223 |
分析证书 | Ab120681 |
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查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 →
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