ExactAb™,已验证,无叠氮钠,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human PDCD6/ALG-2 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF, Flow and so on.
Application:
WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
ICC/IF: 5-20 µg/mL
Flow: 20 µg/mL
Protein Function:
Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity. May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphoprylation of the Akt signaling pathway. Seems to play a role in the regulation of the distribution and function of MCOLN1 in the endosomal pathway. Isoform 2 has a lower Ca(2+) affinity than isoform 1. Isoform 1 and, to a lesser extend, isoform 2, can stabilize SHISA5.
PDCD6/ALG-2 Antibody (Ab120811) - Western Blot
All lanes: PDCD6/ALG-2 Antibody (Ab120811) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 23 kDa
Exposure time: 6.0 s
PDCD6/ALG-2 Antibody (Ab120811) - IF/ICC
FITC staining on IF
Samples: Human HeLa cell
Primary Ab: 20 µg/mL PDCD6/ALG-2 Antibody (Ab120811)
Second Ab: 1 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PDCD6/ALG-2 Antibody (Ab120811) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 μg/mL PDCD6/ALG-2 Antibody (Ab120811)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PDCD6/ALG-2 Antibody (Ab120811) - Flow Cytometry
Human HeLa cell was fixed with 2% paraformaldehyde (10 min), permeabilized with 0.1% BSA-Triton X-100, then stained with 20 µg/mL PDCD6/ALG-2 Antibody (Ab120811) (red histogram) or Isotype control antibody (green histogram), followed by 1 µg/mL FITC-conjugated Anti-rabbit IgG Secondary Antibody.
5-20 µg/mL
5-20 µg/mL
20μg/mL
0.01-2 µg/mL
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0404396 |
分析证书 | Ab120811 |
| ZJ24F0404397 |
分析证书 | Ab120811 |
| ZJ24F0404398 |
分析证书 | Ab120811 |
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