Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
宿主种属
兔(Rabbit)
特异性
PDK1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human PDK1 (AA 15-64)
阳性对照
WB: LNCaP, HeLa, Hep G2, Jurkat, A431, NIH/3T3 and C6 cell lysates.
Recombinant PDK1 Antibody (Ab120985) - Western Blot All lanes: Recombinant PDK1 Antibody (Ab120985) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 45, 70 kDa. The molecular weight observed is consistent with what has been described in PMID: 30171166. Exposure time: 80.0 s
Recombinant PDK1 Antibody (Ab120985) - Western Blot All lanes use Recombinant PDK1 Antibody (Ab120985) at 1/1000 dilution for 1 hour at room temperature.