Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
宿主种属
兔(Rabbit)
特异性
PKM
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant PKM2 expressed in E.coli (AA 141-248).
阳性对照
WB: K562, MCF7, HeLa, Hep G2, Raji, Ramos, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer, skin cancer, prostate, prostate cancer, colorectal cancer, liver cancer, Mouse lung, testis, kidney, oviduct tissues. IF/ICC: MCF7, HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃(1-2周);长期储存-20℃(24个月);收货后建议分装,避免反复冻融。
图片
PKM2 Antibody (Ab122149) - Western Blot All lanes: PKM2 Antibody (Ab122149) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa Observed band size: 61 kDa Exposure time: 3.7 s
PKM2 Antibody (Ab122149) - IF/ICC FITC staining on IF Samples: Human MCF7 cell Primary Ab: 20 μg/mL PKM2 Antibody (Ab122149) Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Skin cancer Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Prostate cancer Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Colorectal cancer Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - Western Blot Lane 1: Wild-type MCF7 cell lysateLane 2: PKM2 knockout MCF7 cell lysatePredicted MW: 56,58 kDaObserved MW: 60 kDaPrimary Ab: 2 µg/mL PKM2 Antibody (Ab122149) Second Ab: 0.2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IF/ICC AF488 staining on IF Samples: HeLa cell Primary Ab: 20 μg/mL PKM2 Antibody (Ab122149) Second Ab: 2 μg/mL AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Mouse Lung Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Control: Used PBS instead of PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Mouse Testis Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Control: Used PBS instead of PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Mouse Oviduct Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Control: Used PBS instead of PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM2 Antibody (Ab122149) - IHC DAB staining on IHC Samples: Mouse Oviduct Tissue Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149) Control: Used PBS instead of PKM2 Antibody (Ab122149) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.