PKM2 Antibody

货号: Ab122149
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 ExactAb™ ? ExactAb™ —— 阿拉丁强调精准和验证质量的抗体系列。适用于可靠、特异性经验证的抗体应用。 无叠氮钠 ? 无叠氮(Azide Free)—— 不含叠氮化钠防腐剂。适用于叠氮有毒或有抑制作用的偶联、细胞工作或检测。 敲低验证 ? 敲低验证(KD)—— 靶标敲低后信号减弱以支持特异性。当需要抗体特异性的功能证据时使用。 已验证 ? 已验证级 —— 发布前在特定应用中确认性能。当需要试剂按声称工作的书面保证时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 见COA
应用
IF/ICC, IHC, WB
宿主种属
兔(Rabbit)
种属反应性
人(Human), 大鼠(Rat), 小鼠(Mouse)
偶联
Unconjugated
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规格
库存
价格
数量
10μl
Ab122149-10μl
现货 Stock Image
¥374.90
50μL
Ab122149-50μl
现货 Stock Image
¥974.90
100μL
Ab122149-100μl
现货 Stock Image
¥1,574.90
1ml
Ab122149-1ml
期货 Stock Image
¥13,999.90
请输入您要添加的产品数量
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为什么选择此级别

ExactAb™,已验证,敲低验证,无叠氮钠,无载体,高性能 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Rabbit anti Human PKM2 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

规格

产品名称
PKM2 Antibody
别名
M2型丙酮酸激酶抗体 | M2型丙酮酸激酶多克隆抗体 | PKM2抗体 | PKM2多克隆抗体 | PKM抗体 | PKM多克隆抗体
英文别名
CTHBP | Cytosolic thyroid hormone-binding protein | MGC3932 | OIP3 | OIP-3 | OIP3EC 2.7.1.40 | Opa-interacting protein 3 | p58 | PK, muscle type | PK2 | PK3 | PK3PKM | PKM2 | Pyruvate kinase 2/3 | pyruvate kinase isozymes M1/M2 | Pyruvate kinase muscle is
规格或纯度
无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 敲低验证, 见COA
生化机理
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
宿主种属
兔(Rabbit)
特异性
PKM
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant PKM2 expressed in E.coli (AA 141-248).
阳性对照
WB: K562, MCF7, HeLa, Hep G2, Raji, Ramos, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer, skin cancer, prostate, prostate cancer, colorectal cancer, liver cancer, Mouse lung, testis, kidney, oviduct tissues. IF/ICC: MCF7, HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃(1-2周);长期储存-20℃(24个月);收货后建议分装,避免反复冻融。
图片

PKM2 Antibody (Ab122149) - Western Blot
All lanes: PKM2 Antibody (Ab122149) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 61 kDa
Exposure time: 3.7 s

PKM2 Antibody (Ab122149) - IF/ICC
FITC staining on IF
Samples: Human MCF7 cell
Primary Ab: 20 μg/mL PKM2 Antibody (Ab122149)
Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - Western Blot
Lane 1: Wild-type MCF7 cell lysateLane 2: PKM2 knockout MCF7 cell lysatePredicted MW: 56,58 kDaObserved MW: 60 kDaPrimary Ab: 2 µg/mL PKM2 Antibody (Ab122149) Second Ab: 0.2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - IF/ICC
AF488 staining on IF
Samples: HeLa cell
Primary Ab: 20 μg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 μg/mL AF488-Linked Caprine Anti-Rabbit IgG Polyclonal
Antibody.

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Lung Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Testis Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Oviduct Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Oviduct Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用
应用名称
稀释比例
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

IF/ICC

5-20µg/mL

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

关联靶点(人)
PKM Tchem 丙酮酸激酶PKM(Pyruvate kinase PKM) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
作用机制
作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到6个结果

批号(Lot Number) 证书类型 货号
ZJ24F0102505 分析证书 Ab122149
ZJ24F0102506 分析证书 Ab122149
ZJ24F0102507 分析证书 Ab122149
ZJ25F0725033 分析证书 Ab122149
ZJ25F0725032 分析证书 Ab122149
ZJ25F0725031 分析证书 Ab122149
技术文档和文章
冷冻样本的免疫组织化学染色实验方案(IHC-Fr)
免疫组织化学染色的抗原修复方案
免疫肽封闭实验
Immunopeptide blocking assay
免疫荧光染色实验步骤(甲醇通透法)
Immunofluorescence Staining Protocol (Methanol permeabilization)
叠氮化钠去除实验
Sodium Azide Removal Protocol
敲低验证(KD)
KD Validation
糖代谢酶月光功能在复制程序、DNA损伤修复与炎症激活中的研究进展
Research Progress on the Moonlighting Functions of Carbohydrate-Metabolic Enzymes in Replication Programs, DNA Damage Repair, and Inflammatory Activation
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Immunohistochemistry (IHC) staining antigen retrieval protocol
糖酵解限速酶调控:己糖激酶、磷酸果糖激酶与丙酮酸激酶的功能比较
Regulation of Glycolytic Rate-Limiting Enzymes: Functional Comparison of Hexokinase, Phosphofructokinase, and Pyruvate Kinase
溶液计算器

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