Rabbit anti Human PSMD9 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on. Application:
WB: 0.01-5 µg/mL IHC: 5-20 µg/mL Flow Cytometry: 5-20 µg/mL per 10^6 cells in 100 µl volume
IF/ICC: 2.5 µg/mL Protein Function: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.
WB: A549, HeLa, Hep G2, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human liver, liver cancer, glioma, lung cancer, breast cancer, prostate tissues. Flow Cytometry: Human SiHa cells. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified + Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4.
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
PSMD9 Antibody (Ab123714) - Western Blot All lanes: PSMD9 Antibody (Ab123714) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 25 kDa Observed band size: 23, 28 kDa Exposure time: 23.7 s
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Lung cancer Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - IHC DAB staining on IHC Samples: Human Prostate Tissue Primary Ab: 10 µg/mL PSMD9 Antibody (Ab123714) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PSMD9 Antibody (Ab123714) - Flow Cytometry Human SiHa cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100, then stained with 20 µg/mL PSMD9 Antibody (Ab123714) (red histogram) or Isotype control antibody (green histogram), followed by 1 µg/mL FITC-conjugated Anti-rabbit IgG Secondary Antibody.
PSMD9 Antibody (Ab123714) - IF/ICC IF analysis of PSMD9 Antibody (Ab123714) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PSMD9 Antibody (Ab123714) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).