Rabbit anti Human Smad1 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function:
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
规格
产品名称
Recombinant Smad1 Antibody
别名
SMAD1抗体|细胞信号转导分子抗体|重组Smad1抗体
英文别名
BSP-1 antibody|BSP1 antibody|HsMAD1 antibody|JV4-1 antibody|JV41 antibody|MAD homolog 1 antibody|MAD mothers against decapentaplegic homolog 1 antibody|Mad related protein 1 antibody|Mad-related protein 1 antibody|MADH1 antibody|MADR1 antibody|Mothers against decapentaplegic homolog 1 antibody|Mothers against DPP homolog 1 antibody|SMA- AND MAD-RELATED PROTEIN 1 antibody|SMAD 1 antibody|SMAD family member 1 antibody|SMAD mothers against DPP homolog 1 antibody|Smad1 antibody|TGF beta signaling protein 1 antibody|Transforming growth factor-beta-signaling protein 1 antibody|BSP1|BSP-1|hSMAD1|SMAD, mothers against DPP homolog 1 (Drosophila)|MAD homolog 1|MADH1|JV41|MADR1|MAD, mothers against decapentaplegic homolog 1 (Drosophila)|Mad-related protein 1|mothers against decapentaplegic homolog 1|Mothers against DPP homolog 1|SMAD family member 1|SMAD 1|SMAD, mothers against DPP homolog 1|Smad1|TGF-beta signaling protein 1|transforming growth factor-beta signaling protein 1|Transforming growth factor-beta-signaling protein 1
规格或纯度
ExactAb™, Validated, 重组, 0.8 mg/mL
宿主种属
兔(Rabbit)
特异性
SMAD1
种属反应性
人(Human)
免疫原
A synthetic peptide derived from human Smad1 (AA 220-280).
阳性对照
WB: Hep G2, HEK293, HUVEC and HeLa cell lysates. IHC: Human colon tissue. IF/ICC: HeLa cells.
Recombinant Smad1 Antibody (Ab128163) - Western Blot All lanes: Recombinant Smad1 Antibody (Ab128163) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 52 kDa Observed band size: 57 kDa Exposure time: 45.1 s
Recombinant Smad1 Antibody (Ab128163) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon using Recombinant Smad1 Antibody (Ab128163). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant Smad1 Antibody (Ab128163) - IF/ICC
IF analysis of Recombinant Smad1 Antibody (Ab128163) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Smad1 Antibody (Ab128163) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
应用
应用名称
稀释比例
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/200
高级数据
关联靶点（人）
SMAD1Tbio母亲反对 decapentaplegic 同源物 1(Mothers against decapentaplegic homolog 1)
(0 活性数据)
ZJ24F0303518
危险品化学品经营许可证（带存储）