This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Post-translational: Phosphorylation on Tyr-10 modulates pumping activity.
宿主种属
兔(Rabbit)
特异性
ATP1A1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Sodium potassium ATPase (AA 35-80).
阳性对照
WB: HeLa, A375, Hep G2, SH-SY5Y, NIH/3T3 and C6 cell lysates. IHC: Rat cerebral cortex, heart, Human cervical cancer, thyroid cancer, thyroid carcinoma, Mouse skeletal muscle-gastrocnemius tissue. IF/ICC: HeLa cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Sodium Potassium ATPase/ATP1A1 Antibody (Ab128630) - Western Blot All lanes: Recombinant Sodium Potassium ATPase/ATP1A1 Antibody (Ab128630) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 113 kDa Observed band size: 91 kDa Exposure time: 50.0 s
Recombinant Sodium Potassium ATPase/ATP1A1 Antibody (Ab128630) - IHC Immunohistochemical analysis of paraffin-embedded Rat heart, using the Recombinant Sodium Potassium ATPase/ATP1A1 Antibody (Ab128630) at 1/100 dilution.
Recombinant Sodium Potassium ATPase/ATP1A1 Antibody (Ab128630) - IHC Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Recombinant Sodium Potassium ATPase/ATP1A1 Antibody (Ab128630) at 1/100 dilution.
Recombinant Sodium Potassium ATPase/ATP1A1 Antibody (Ab128630) - IF/ICC IF analysis of Recombinant Sodium Potassium ATPase/ATP1A1 Antibody (Ab128630) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Sodium Potassium ATPase/ATP1A1 Antibody (Ab128630) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ25F0420717
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