ExactAb™,已验证,重组,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human SOX10 Antibody, Recombinant, could be used for WB, IHC, IF/ICC, Flow Cytometry, IP and so on.
Recombinant SOX10 Antibody (Ab128734) - Western Blot
All lanes: Recombinant SOX10 Antibody (Ab128734) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 57, 70 kDa
Exposure time: 1.1 s
Recombinant SOX10 Antibody (Ab128734) - IF/ICC
Recombinant SOX10 Antibody (Ab128734) staining SOX10 in A375 cells by IF/ICC. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant SOX10 Antibody (Ab128734) (1/10000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An AF488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant SOX10 Antibody (
Ab128734
) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling SOX10 with Recombinant SOX10 Antibody (Ab128734) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant SOX10 Antibody (Ab128734) - IP
SOX10 was immunoprecipitated from 0.4 mg of A375 cell lysate with Recombinant SOX10 Antibody (Ab128734) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant SOX10 Antibody (Ab128734) IP in A375 whole cell lysate
Lane 2: Rabbit IgG instead of Recombinant SOX10 Antibody (Ab128734) in A375 whole cell lysate
Lane3: A375 whole cell lysate, 10 μg (input)
Exposure: 120.0 s.
Recombinant SOX10 Antibody (Ab128734) - Flow Cytometry
Overlay histogram showing A375 cells stained with Recombinant SOX10 Antibody (Ab128734)(blue). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant SOX10 Antibody (Ab128734) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (red) was used as a control.
1/50
1/200 - 1/1000
1/1000 - 1/2000
1/1000 - 1/2000
1/2000 - 1/10000
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ23F1201987 |
分析证书 | Ab128734 |
| ZJ23F1201988 |
分析证书 | Ab128734 |
| ZJ25F0420044 |
分析证书 | Ab128734 |
| ZJ24F1011888 |
分析证书 | Ab128734 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
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