Rabbit anti Human TOMM40 Antibody, Polyclonal, could be used for WB, ICC, IF, Flow and so on. Application: WB: 1/2000 Flow: 1/25 ICC/IF: 1/25 Protein Function Channel-forming protein essential for import of protein precursors into mitochondria.
规格
产品名称
TOMM40 Antibody
别名
TOMM40 抗体 | TOMM40抗体
英文别名
p38.5 | Protein Haymaker | Translocase of outer membrane 40 kDa subunit homolog | C19orf1 antibody | D19S1177E antibody | Haymaker protein antibody | Mitochondrial import receptor subunit TOM40 homolog antibody | Mitochondrial outer membrane protein antib
规格或纯度
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
宿主种属
兔(Rabbit)
特异性
TOMM40
种属反应性
人(Human)
免疫原
A KLH conjugated synthetic peptide from human TOMM40 (AA 22-56)
阳性对照
WB: HEK293, HeLa, A431, Hep G2 and SK-OV-3 cell lysates. ICC/IF: U2OS cells. Flow: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Protein A purified + Peptide affinity purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 0.09% Sodium azide, pH 7.4.
防腐剂
0.09% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存（2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
图片
TOMM40 Antibody (Ab131963) - Western Blot All lanes: TOMM40 Antibody (Ab131963) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 38 kDa Observed band size: 38, 39 kDa Exposure time: 12.9 s
TOMM40 Antibody (Ab131963) - IF/ICC Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U2OS (human osteosarcoma cell line) cells labeling TOMM40 with TOMM40 Antibody (Ab131963) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on U2OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
TOMM40 Antibody (Ab131963) - Flow Cytometry Overlay histogram showing HeLa cells stained with TOMM40 Antibody (Ab131963) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the TOMM40 Antibody (Ab131963) (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
ZJ24F0403966
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