ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human DDX39B Antibody, Polyclonal, could be used for WB, IHC, Flow and so on.
Application:
· WB: 1/1000
· IHC: 1/100-1/500
· Flow: 1/10-1/50
Protein Function
Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.
UAP56 Antibody (Ab133187) - Western Blot
All lanes: UAP56 Antibody (Ab133187) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 35.4 s
UAP56 Antibody (Ab133187) - Western Blot
All lanes: UAP56 Antibody (Ab133187) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 16.5 s
UAP56 Antibody (Ab133187) - IHC
Formalin-fixed and paraffin-embedded human kidney reacted with UAP56 Antibody (Ab133187), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
UAP56 Antibody (Ab133187) - Flow Cytometry
UAP56 Antibody (Ab133187) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
1/1000
1/100-1/500
1/10-1/50
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0303481 |
分析证书 | Ab133187 |
| ZJ24F0303482 |
分析证书 | Ab133187 |
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