ExactAb™,PBS Only,已验证,无叠氮钠,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Application
WB: 1/2,000-1/10,000 ; Predicted molecular weight: 54 kDa
Flow: 0.008 μg/mL – 1 μg/mL
ICC/IF: 2 μg/mL
Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 55.4 kDa
Exposure time: 25.0 s
Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 55.5 kDa
Exposure time: 25.0 s
Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 55.4 kDa
Exposure time: 10.1 s
Vimentin Mouse mAb (Ab179444) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab179444) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
Vimentin Mouse mAb (Ab179444) - ICC/IF
IF analysis of Vimentin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vimentin Mouse mAb (Ab179444) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
2 μg/mL
0.008-1μg/mL
1/2,000-1/10,000 ; Predicted molecular weight: 54 kDa
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0102679 |
分析证书 | Ab179444 |
| ZJ24F0102678 |
分析证书 | Ab179444 |
| ZJ24F0102677 |
分析证书 | Ab179444 |
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