CD235a Mouse mAb (FITC)

    应用:
  • Flow Cytometry
功能和特点
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG2b
  • 克隆号: HIR2
  • 偶联: FITC
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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab179692-10T (试用装)
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10T 现货 Stock Image
Ab179692-25T
25T 现货 Stock Image
Ab179692-100T
100T 现货 Stock Image

基本信息

产品名称 CD235a Mouse mAb (FITC)
别名 异硫氰酸荧光素标记的小鼠抗人CD235a抗体 | CD235a 小鼠单克隆抗体 (FITC) | CD235a 小鼠单抗 (FITC)
英文别名 MN sialoglycoprotein | PAS-2 | Sialoglycoprotein alpha | CD235a antigen | CD235a | glycophorin A (includes MN blood group) | glycophorin A (MNS blood group) | Glycophorin A | glycophorin Erik | glycophorin MiI | glycophorin MiIII | glycophorin MiV | glyco
规格或纯度 ExactAb™, 已验证, 无叠氮钠, Ex:498nm, Em:517nm, 5μL/test
宿主种属 小鼠(Mouse)
特异性 GYPA
种属反应性 人(Human)
交叉反应 GYPB
免疫原 Synthetic peptide (Human, N-terminal)
阳性对照 Flow: K562
偶联 FITC
Excitation(nm) Blue Laser(488nm)
Emission(nm) 517nm
Ex/Em(nm) Ex:498nm;Em:517nm

AI解读

产品属性

克隆类型 单克隆抗体
克隆号 HIR2
Format Whole IgG
亚型 Mouse IgG2b
轻链亚型 kappa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂 0.05% Proclin300
浓度 5μL/test
储存温度 2-8°C储存,避光,禁止冻结
运输条件 冰袋运输
稳定性与储存 未稀释的抗体溶液应保存在 2°C 至 8°C 之间,避免长时间暴露在光线下。切勿冷冻。
分子类型 抗体

图片

CD235a Mouse mAb (FITC) (Ab179692) - Flow Cytometry
Flow cytometry analysis of CD235a expression by K562 cells (red) compared to an isotype control (blue). Cells were stained with CD235a Mouse mAb (FITC) (Ab179692) at 0.3μg/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.

关联靶点(人)

GYPA Tbio 血型糖蛋白-A(Glycophorin-A) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

应用

应用名称 稀释比例
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到3个结果

批号(Lot Number) 证书类型 日期 货号
ZJ24F0303127 分析证书 24-03-12 Ab179692
ZJ24F0303128 分析证书 24-03-12 Ab179692
ZJ24F0303129 分析证书 24-03-12 Ab179692

参考文献

1. Bruce LJ, Pan RJ, Cope DL, Uchikawa M, Gunn RB, Cherry RJ, Tanner MJ. Altered structure and anion transport properties of band 3 (AE1, SLC4A1) in human red cells lacking glycophorin A..  J Biol Chem,  [PMID:14604989] [http://pubmed.ncbi.nlm.nih.gov/14604989]
2. Tomita, M M and Marchesi, V T VT..  (1975)  Amino-acid sequence and oligosaccharide attachment sites of human erythrocyte glycophorin..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:1059087]
3. Huang, C H CH, Skov, F F, Daniels, G G, Tippett, P P and Blumenfeld, O O OO..  (1992)  Molecular analysis of human glycophorin MiIX gene shows a silent segment transfer and untemplated mutation resulting from gene conversion via sequence repeats..  Blood,  (1): [PMID:1421409]
4. Huang, C H CH, Spruell, P P, Moulds, J J JJ and Blumenfeld, O O OO..  (1992)  Molecular basis for the human erythrocyte glycophorin specifying the Miltenberger class I (MiI) phenotype..  Blood,  (1): [PMID:1611092]
5. Hamid, J J and Burness, A T AT..  (1990)  The mechanism of production of multiple mRNAs for human glycophorin A..  Nucleic acids research,  (11): [PMID:2216775]
6. Dill, K K, Hu, S H SH, Berman, E E, Pavia, A A AA and Lacombe, J M JM..  (1990)  One- and two-dimensional NMR studies of the N-terminal portion of glycophorin A at 11.7 Tesla..  Journal of protein chemistry,  [PMID:2386609]
7. Kudo, S S and Fukuda, M M..  (1989)  Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2734312]
8. Tate, C G CG and Tanner, M J MJ..  (1988)  Isolation of cDNA clones for human erythrocyte membrane sialoglycoproteins alpha and delta..  The Biochemical journal,  (15): [PMID:3196288]
9. Rahuel, C C and 8 more authors..  (1988)  Characterization of cDNA clones for human glycophorin A. Use for gene localization and for analysis of normal of glycophorin-A-deficient (Finnish type) genomic DNA..  European journal of biochemistry,  (15): [PMID:3345758]
10. Siebert, P D PD and Fukuda, M M..  (1986)  Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:3456608]
11. Siebert, P D PD and Fukuda, M M..  (1986)  Molecular biological study of the structure and expression of human glycophorin A..  Revue francaise de transfusion et immuno-hematologie,  [PMID:3809885]
12. Pasvol, G G, Wainscoat, J S JS and Weatherall, D J DJ..  (1982)  Erythrocytes deficiency in glycophorin resist invasion by the malarial parasite Plasmodium falciparum..  Nature,  (6): [PMID:7040988]
13. Kudo, S S, Onda, M M and Fukuda, M M..  (1994)  Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals..  Journal of biochemistry,  [PMID:7798177]
14. Huang, C H CH, Reid, M M, Daniels, G G and Blumenfeld, O O OO..  (1993)  Alteration of splice site selection by an exon mutation in the human glycophorin A gene..  The Journal of biological chemistry,  (5): [PMID:8245024]
15. Pisano, A A, Redmond, J W JW, Williams, K L KL and Gooley, A A AA..  (1993)  Glycosylation sites identified by solid-phase Edman degradation: O-linked glycosylation motifs on human glycophorin A..  Glycobiology,  [PMID:8286855]
16. Adams, P D PD, Engelman, D M DM and Brünger, A T AT..  (1996)  Improved prediction for the structure of the dimeric transmembrane domain of glycophorin A obtained through global searching..  Proteins,  [PMID:8953647]
17. MacKenzie, K R KR, Prestegard, J H JH and Engelman, D M DM..  (1997)  A transmembrane helix dimer: structure and implications..  Science (New York, N.Y.),  (4): [PMID:9082985]
18. Poole, J and 7 more authors..  (1999)  Glycophorin A mutation Ala65 --> Pro gives rise to a novel pair of MNS alleles ENEP (MNS39) and HAG (MNS41) and altered Wrb expression: direct evidence for GPA/band 3 interaction necessary for normal Wrb expression..  Transfusion medicine (Oxford, England),  [PMID:10354388]
19. Storry, J R JR, Coghlan, G G, Poole, J J, Figueroa, D D and Reid, M E ME..  (2000)  The MNS blood group antigens, Vr (MNS12) and Mt(a) (MNS14), each arise from an amino acid substitution on glycophorin A..  Vox sanguinis,  [PMID:10729812]
20. Young, Mark T MT and Tanner, Michael J A MJ..  (2003)  Distinct regions of human glycophorin A enhance human red cell anion exchanger (band 3; AE1) transport function and surface trafficking..  The Journal of biological chemistry,  (29): [PMID:12813056]
21. Yajima, Ayako A and 5 more authors..  (2008)  Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane..  Microbiology and immunology,  [PMID:18380804]
22. Pang, Allison J AJ and Reithmeier, Reinhart A F RA..  (2009)  Interaction of anion exchanger 1 and glycophorin A in human erythroleukaemic K562 cells..  The Biochemical journal,  (15): [PMID:19438409]

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