计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
EnzymoPure™,天然 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 3 篇同行评审文献引用。
概述
基因名： CA1; CA2
基因 ID：510801; 280740
Carbonic anhydrase (CA) catalyzes the reversible hydration and dehydration reactions of CO2/H2CO3. CAs are widespread in nature, being found in animals, plants, and certain bacteria. Sixteen isozymes have been identified and characterized in mammals. Since erythrocyte CA is relatively easy to obtain for experimental purposes, it is the most widely studied. Specificity Blood CO2 transport and excretion is largely dependent on the rapid catalysis of the CO2 reactions within the erythrocyte by CA. Bovine CA reversibly hydrates alkyl pyruvates and it exhibits hydratase activity toward a wide variety of substrates. Composition Sixteen CA isozymes have been described so far in mammals. Erythrocyte CAs, CA-I and CA-II, are most well known. CA-I, CA-II, CA-III, CA-VII, and CA-XIII are cytosolic. CA-IV, CA-IX, CA-XII, CA-XIV, and CA-XV are membrane bound. CA-VI is secreted in saliva. CA-VA and CA-VB are mitochondrial. There are also three acatalytic forms referred to as CA-related proteins (CARPs): CARP-VIII, CARP-X, and CARP-XI. The zinc metal is always bound to histidines 93, 95, and 118 (mature chain numbering). A hydrogen bonded network, linked to the zinc-bonded water molecule and these histidines either directly or indirectly, includes 28-Ser, 91-Glu, 105-Glu, 106-His, 116-His, 193-Tyr, 198-Thr, 208-Trp, and 223-Asn. These residues have been found to be highly conserved. Bovine and human CA I and II contain a unique C-terminal knot structure, which has been shown to be important in enzymatic and mechanical properties. Molecular Characteristics Each isozyme from erythrocytes (CA-I and CA-II) is composed of a single chain peptide of 259 or 260 amino acid residues. The low activity form (CA-I) contains 260 residues, while the high activity form (CA-II) contains 259 residues. Erythrocyte high and low activity forms within a given species usually show greater than 50% sequence identity; for example, equine CA-I and CA-II forms only show 55% identity. In contrast, the same forms from different species show much greater homology; human CA-II and bovine CA-II show 77% sequence homology. The isozymes are encoded by separate genes but given the great deal of homology, especially in the active center, they appear to have a common evolutionary history. Ubiquitin, a 76 residue protein with some enzymatic properties of CAs, has a distinct sequence homology to CA. Applications CO2 determination in blood Elimination of CO2 in reagents for acidity testing Carboxy group transfers Reduction reactions Carbonic Anhydrase Assay Method The electrometric method of Wilbur and Anderson (1948) in which the time required (in seconds) for a saturated CO2 solution to lower the pH of 0.012 M Tris⋅HCl buffer from 8.3 to 6.3 at 0°C is determined. The time without enzyme is recorded at T0; with enzyme, T. Reagents 0.02 M Tris⋅HCl buffer, pH 8.0. Store in an ice bath at 0-4°C before and during use. Carbon dioxide saturated water. Bubble CO2 gas through 200 ml ice cold water for 30 minutes prior to assay. During saturation process, store water at 0-4°C in an ice bath. Enzyme Dissolve lyophilized powder at a concentration of 0.1 mg/ml in ice cold water. Store in ice bath prior to use. IMMEDIATELY prior to use dilute suspensions or lyophilized materials to a concentration of approximately 0.01 mg/ml in ice cold water. Procedure Blank Determination: Add 6.0 ml of chilled 0.02 M Tris⋅HCl buffer, pH 8.0 to a 15-20 ml beaker. Maintain temperature at 0-4°C and record pH. Withdraw in a 5 ml syringe, 4 ml of chilled CO2 saturated water and add to Tris buffer. Immediately start a stop watch and record the time required for the pH to drop from 8.3 to 6.3. Record this time as T0. Enzyme Determination: Add 6.0 ml of chilled 0.02 M Tris⋅HCl buffer, pH 8.0 to a 20 ml beaker. Maintain temperature at 0-4°C and record pH. Add 0.1 ml of freshly diluted enzyme. Quickly add 4 ml of CO2 saturated water and record the time required for the pH to drop from 8.3 to 6.3. Record this time as T. Calculation
规格
产品名称
碳酸酐酶
英文别名
Carbonate Dehydratase | Carbonate Hydrolyase
规格或纯度
天然, EnzymoPure™, ≥3,000 units/mg dry weight; from bovine erythrocytes
生化机理
碳酸酐酶是一种锌金属酶，分子量约为 30,000 Da。该酶催化二氧化碳水合为碳酸。它参与重要的生理和病理过程，如 pH 值和二氧化碳的平衡、碳酸氢盐和二氧化碳的运输、生物合成反应、骨吸收、钙化和肿瘤生成。因此，这种酶是抑制剂的一个重要靶点，临床应用于各种病症，如青光眼、癫痫和帕金森病。
生物活性
≥3,000 units/mg dry weight
种属
牛(Bovine)
来源
天然
CAS编号和信息
9001-03-0
酶学委员会编号
4.2.1.1
分子类型
酶
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥3,000 units/mg dry weight; from bovine erythrocytes
储存条件
2-8°C储存
运输条件
低温运输
单位定义
One Unit is determined by the electrometric method of Wilbur and Anderson (J. Biol. Chem., 176, 147 (1948)), in which the time required (in seconds) for a saturated CO2 solution to lower the pH of 0.02M Tris-HCl buffer from 8.3 to 6.3, at 0-4°C is determi
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 H2627066 |
分析证书 | C128742 |
| H2627023 |
分析证书 | C128742 |
| H2627022 |
分析证书 | C128742 |
| H2627014 |
分析证书 | C128742 |
| A2626452 |
分析证书 | C128742 |
| A2626815 |
分析证书 | C128742 |
| A2626433 |
分析证书 | C128742 |
| J2515340 |
分析证书 | C128742 |
| J2515302 |
分析证书 | C128742 |
| H2522772 |
分析证书 | C128742 |
| H2520660 |
分析证书 | C128742 |
| C2507322 |
分析证书 | C128742 |
| C2507323 |
分析证书 | C128742 |
| E2431091 |
分析证书 | C128742 |
| E2431104 |
分析证书 | C128742 |
| E2431093 |
分析证书 | C128742 |
| E2431092 |
分析证书 | C128742 |
| E2431090 |
分析证书 | C128742 |
| D2403171 |
分析证书 | C128742 |
| F2518172 |
分析证书 | C128742 |
| F2518195 |
分析证书 | C128742 |
| C2318947 |
分析证书 | C128742 |
| J2311119 |
分析证书 | C128742 |
| C2318945 |
分析证书 | C128742 |
| G2207196 |
分析证书 | C128742 |
| G2207197 |
分析证书 | C128742 |
| B2314433 |
分析证书 | C128742 |
技术文档和文章
此产品的引用文献
溶液计算器
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