Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification, and bone resorption. Topics in the CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in the clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis, and cerebral calcification.
生物活性
Measured by its esterase activity. The specific activity is >100 pmol/min/µg, as measured under the described conditions.
29.2 kDa, under reducing conditions; 29.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
20mM Tris, 500mM NaCl, 10% Glycerol, pH 8.0
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Carbonic Anhydrase II Protein (rp183662) - SDS-PAGE 3 μg/lane of Recombinant Human Carbonic Anhydrase II Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 29.2 kDa.
Recombinant Human Carbonic Anhydrase II Protein- Enzyme Activity Measured by its esterase activity. The specific activity is >100 pmol/min/µg, as measured under the described conditions. Conditions: Assay Buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5 Recombinant Human Carbonic Anhydrase II Protein (rp183662) Substrate: 4-Nitrophenyl Acetate (4-NPA) (N111060) 1. Dilute rhCA2 to 2 ng/µL in Assay Buffer. 2. Dilute Substrate to 2 mM in Assay Buffer. 3. In a plate, load 50 µL of 2 ng/µL Recombinant Human Carbonic Anhydrase II Protein (rp183662), and start the reaction by adding 50 µL of 2 mM Substrate to wells. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 2 mM Substrate. 4. Read absorbance at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
ZJ25F0319143
危险品化学品经营许可证（带存储）