计算溶液所需的质量、体积或浓度。
鼠尾 I 型胶原蛋白
有货
为什么选择此级别
无菌,用于细胞培养 级 提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,禁止冷冻。低温运输,禁止冷冻 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
鼠尾I型胶原蛋白( Collagen from rat tail, Type I)系采用Birkedal-Hansen方法在无菌条件下制备，纯度达到95%以上，溶于0.006mol/L乙酸，浓度4.5~5.5mg/mL。本品可用于细胞培养器皿的包被，特别适合普通细胞培养器皿不易贴壁细胞的培养；也可用于制备三维胶原凝胶，使细胞在模拟的三维环境中生长。
质量标准
1、SDS-PAGE分析纯度大于95%。
2、无菌检验结果为阴性。
3、本品2ug/cm<sup>2</sup>包被细胞培养皿后培养PC-12细胞，贴壁及生长正常。
4、本品浓度1mg/ml，pH7.0时形成具有一定强度的三维胶原凝胶，NIH-3T3细胞在三维凝胶内生长正常、PC-12细胞在三维胶表面生长正常。
使用方法
1、细胞培养器皿的表面包被
以包被浓度为2ug/cm<sup>2</sup>为例，用无菌0.006mol/L乙酸将胶原蛋白稀释到0.012mg/ml。按下表体积加到相应的培养器皿中，确保胶原蛋白溶液铺满器皿的表面。开盖在超净台上过夜晾干，也可以在室温放置1小时后，用PBS洗3-4次后直接使用。包被好的器皿在4-25ºC至少可保存3个月以上的时间。
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2、三维胶原凝胶的制备
A、无细胞三维胶原凝胶的制备（以配制1mg/ml三维胶1ml为例）
将200µl本品加到置于冰浴的离心管中，加入690µl双蒸水，然后加到12µl 0.1mol/L NaOH中（如果反过来把12µl 0.1mol/L NaOH加到胶原溶液中，会由于NaOH不能迅速混匀而产生局部的胶原凝结），立即混匀。再加入100µl 10xPBS或10x培养液，混匀后立即加到培养器皿中(混匀后pH为7左右，如果PBS或培养液中没有加酚红，初次使用时需要测定pH值)。将培养器皿在室温放置20min待胶凝固后，转移到培养箱内。如果配制中使用的是10xPBS，使用前需要加入适当体积的细胞培养液预平衡。
B、含细胞三维胶原凝胶的制备（以配制1mg/ml三维胶1ml为例）
准备好悬浮于培养液的细胞，并放置于冰浴中。将200µl本品加到12µl 0.1mol/L NaOH中（如果反过来把12µl 0.1mol/L NaOH加到胶原溶液中，会由于NaOH不能迅速混匀而产生局部的胶原凝结），立即混匀。再加入23µl 10xPBS或10x培养液，混匀(混匀后pH为7左右，如果PBS或培养液中没有加酚红，初次使用时需要测定pH值)。加入760µl的细胞悬浮液，混匀后立即加到培养器皿中。将培养器皿在室温下放置20min待胶凝固后，加入适当体积的细胞培养液，转移到培养箱中培养。本品在室温下pH中性时可迅速成胶，在操作过程中要尽量保持低温。
Collagen from rat tail, Type I is prepared under aseptic conditions using the Birkedal-Hansen method, with a purity of ≥95%. It is dissolved in 0.006 mol/L acetic acid at a concentration of 4–5 mg/mL. This product can be used for coating cell culture vessels, especially for culturing cells that are difficult to adhere on ordinary cell cultureware. It can also be used to prepare three-dimensional collagen gels, allowing cells to grow in a simulated three-dimensional environment.
Quality Standards
1.Purity >95% analyzed by SDS-PAGE.
2.Sterility test result is negative.
3.After coating cell culture dishes at 2 μg/cm<sup>2</sup>, PC-12 cells show normal adhesion and growth.
4.At a concentration of 1 mg/mL and pH 7.0, this product forms 3D collagen gels with adequate strength. NIH-3T3 cells grow normally inside the 3D gels, and PC-12 cells grow normally on the surface of the 3D gels.
Directions for Use
1. Surface Coating of Cell Culture Vessels
Take a coating concentration of 2 μg/cm <sup>2</sup> as an example. Dilute the collagen to 0.012 mg/mL with sterile 0.006 mol/L acetic acid. Add the volume listed in the table below to the corresponding culture vessel, ensuring the collagen solution covers the entire surface. Remove the cap and air-dry overnight in a laminar flow hood. Alternatively, leave at room temperature for 1 hour, wash 3–4 times with PBS, and use immediately. Coated vessels can be stored at 4–25 °C for at least 3 months.
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2. Preparation of 3D Collagen Gels
A. Preparation of Cell-Free 3D Collagen Gels
(Example: 1 mL of 1 mg/mL 3D gel)Add 200 μL of this product to a centrifuge tube in an ice bath. Add 690 μL of double-distilled water, then add to 12 μL of 0.1 mol/L NaOH.Note: Adding 12 μL of 0.1 mol/L NaOH into the collagen solution may cause local collagen gelation due to uneven mixing.Mix immediately. Add 100 μL of 10×PBS or 10×culture medium, mix well, and add to the culture vessel immediately.The pH will be approximately 7 after mixing. If phenol red is not included in PBS or medium, measure pH upon first use.Leave at room temperature for 20 min until gelation is complete, then transfer to an incubator. If 10×PBS is used, pre-equilibrate with an appropriate volume of cell culture medium before use.
B. Preparation of Cell-Containing 3D Collagen Gels
(Example: 1 mL of 1 mg/mL 3D gel)Prepare cells suspended in culture medium and keep in an ice bath. Add 200 μL of this product to 12 μL of 0.1 mol/L NaOH.Note: Adding 12 μL of 0.1 mol/L NaOH into the collagen solution may cause local collagen gelation due to uneven mixing.Mix immediately. Add 23 μL of 10×PBS or 10×culture medium and mix well.The pH will be approximately 7 after mixing. If phenol red is not included in PBS or medium, measure pH upon first use.Add 760 μL of cell suspension, mix gently, and add to the culture vessel immediately. Leave at room temperature for 20 min until gelation is complete. Add an appropriate volume of cell culture medium and transfer to an incubator.This product gels rapidly at neutral pH and room temperature. Keep samples cold during all procedures.
规格
产品名称
鼠尾 I 型胶原蛋白
别名
鼠尾 Collagen from rat tail, Type I 蛋白 | 鼠尾胶原蛋白 I 型
英文别名
Collagen, Type I, from Rat Tail | Collagen Type I (Rat tail) | Collagen I from Rat Tail
规格或纯度
用于细胞培养, 无菌, ≥95%(SDS-PAGE), 4.5-5.5mg/mL
生化机理
Type I collagen is a member of group I collagen (fibrillar forming collagen).
种属
大鼠(Rat)
无载体
No
无动物源
No
Accession #
来源
天然
分子类型
蛋白质
储存与运输
物理形态
液体
储存缓冲液
0.006mol/L Acetic acid
浓度
4.5-5.5mg/mL
储存条件
2-8°C储存,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
长期储存4℃（36个月）收货后建议分装，切勿冻存
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0736964 |
分析证书 | C1523053 |
技术文档和文章
Culture Systems, Molecular Markers, and Research Applications of Major Human Organ Organoids
目录： 技术文章
Culture Systems, Phenotypic Characterization, and Translational Research Applications of Tumor Organoids
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