计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
组织蛋白酶 B(CTSB),由 CTSB 基因编码,属于溶酶体半胱氨酸蛋白酶家族, 在细胞内蛋白水解中起着重要作用。它在新陈代谢过程中在肌肉组织中产生。 它可以增强其他蛋白酶的活性,包括基质金属蛋白酶,尿激酶(丝氨酸蛋白酶尿激酶纤溶酶酶激活剂)和组织蛋白酶 D,因此它对细胞外基质组分的蛋白水解,细胞间通讯破坏和蛋白酶抑制剂表达降低具有重要作用。它还参与自噬和分解代谢,这在肿瘤恶性肿瘤中是有利的,并且可能参与特定的免疫抵抗。它的过表达与浸润性和转移性癌症相关。多种疾病导致 CTS-B 水平升高,导致许多病理过程,包括细胞死亡、炎症和有毒肽的产生。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人组织蛋白酶B(CTSB)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人组织蛋白酶B(CTSB)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CTSB. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CTSB present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.