β-Glucuronidase is a glycoside hydrolase that catalyzes the hydrolysis of β-D-glucuronide bonds, releasing glucuronic acid and the corresponding aglycone.
SPECIFICTY: Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates.
生物活性,重组,ActiBioPure™,高性能,EnzymoPure™,≥5000 U/mg enzyme powder; ≥30000 U/mg protein
生化机理
Displays beta-glucuronidase activity with the artificial substrate p-nitrophenyl-beta-D-glucuronide (PNPG) and with 4-methylumbelliferyl-glucuronide. Is likely capable of scavenging glucuronate from a range of chemically distinct xenobiotic and endobiotic glucuronides present in the gastrointestinal (GI) tract, to be able to utilize these diverse sources of carbon. As part of the GI microbiome, this enzyme is able to reactivate glucuronide drug conjugates, such reactivated compounds can significantly damage the GI tract. Catalytic activity: a beta-D-glucuronoside + H2O = D-glucuronate + an alcohol. This reaction proceeds in the forward direction. EC:3.2.1.31. Rhea:76111: 4-methylumbelliferone beta-D-glucuronate + H2O = 4-methylumbelliferone + D-glucuronate: This reaction proceeds in the forward direction. Activity regulation: Potently inhibited by a set of synthetic compounds like thio-urea derivatives and analogs, and uronic isofagomine (UIFG) derivatives. Inhibitors of gut microbial beta-glucuronidases block the reactivation of glucuronidated cancer drugs, and thereby alleviate drug-induced GI toxicity. Kinetics: kcat is 120 sec-1 with p-nitrophenyl-beta-D-glucuronide as substrate. pH Dependence: Optimum pH is 5.0-7.5. Temperature Dependence: Resistant to thermal inactivation at 50 degrees Celsius.
生物活性
≥5000 U/mg enzyme powder; ≥30000 U/mg protein
N-末端序列
One unit will liberate 1.0 microgram of phenolphthalein from phenolphthaleinglucuronide per hour at pH 6.8 at 37℃ (30min assay).
来源
重组表达
CAS编号和信息
9001-45-0
酶学委员会编号
EC 3.2.1.31
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥5000 U/mg enzyme powder; ≥30000 U/mg protein
复溶
Reconstitute in water to a concentration of <5 mg/ml.
ZJ26F0939220
危险品化学品经营许可证（带存储）