计算溶液所需的质量、体积或浓度。
BioReagent,无菌过滤,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
ITS-X Media Supplement作为基础培养基补充剂,以减少培养细胞所用的胎牛血清 (FBS) 的量。ITS-X溶液通常与2–4% FBS联合使用, 以支持需要乙醇胺的细胞类型,如支气管上皮细胞。我们还提供ITS-G和ITS-A Media Supplement。
组分表:
| Component (mg/L) | I747211 ITS-G Media Supplement | I747212 ITS-A Media Supplement | I747213 ITS-X Media Supplement |
| Insulin | 1000 | 1000 | 1000 |
| Transferrin | 550 | 550 | 550 |
| Sodium Selenite | 0.67 | 0.67 | 0.67 |
| Sodium Pyruvate | / | 11000 | / |
| Ethanolamine | / | / | 200 |
胰岛素可促进葡萄糖和氨基酸摄取、脂肪形成、细胞内运输以及蛋白和核酸的合成。转铁蛋白是铁载体,也有助于降低氧自由基和过氧化物的毒性水平。硒 (以亚硒酸钠提供) 是谷胱甘肽过氧化物酶和其他蛋白的一种辅因子,在培养基中用作抗氧化剂。乙醇胺是磷酸甘油酯的前体,对质膜和细胞器的结构至关重要。
使用说明:
按照100×浓度将本产品加入培养基,一瓶可以配置1L的培养基。培养初期培养基中需加入2%~4%的胎牛血清,待细胞适应后,胎牛血清浓度可进一步降低。
注意事项:
1、本产品经0.22μm过滤除菌。
2、使用本产品时应注意无菌操作,避免污染。
3、本产品为100×浓缩液,请根据需要稀释后使用。
4、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
物理外观:Liquid
储存缓冲液:EBSS
是否无菌:是
ITS-X Media Supplement is used as a basal medium additive to reduce the required amount of fetal bovine serum (FBS) for cell culture. Typically combined with 2–4% FBS, it supports the culture of cell types that require ethanolamine, such as bronchial epithelial cells. We also supply ITS-G and ITS-A Media Supplement.
Composition Table:
| Component (mg/L) | I747211 ITS-G Media Supplement | I747212 ITS-A Media Supplement | I747213 ITS-X Media Supplement |
| Insulin | 1000 | 1000 | 1000 |
| Transferrin | 550 | 550 | 550 |
| Sodium Selenite | 0.67 | 0.67 | 0.67 |
| Sodium Pyruvate | / | 11000 | / |
| Ethanolamine | / | / | 200 |
Insulin promotes the uptake of glucose and amino acids, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin acts as an iron carrier and helps mitigate the toxicity of oxygen free radicals and peroxides. Selenium, provided in the form of sodium selenite, functions as a cofactor for glutathione peroxidase and other proteins, and serves as an antioxidant in culture media. Ethanolamine is a precursor of phosphoglycerides and is vital for maintaining the structure of plasma membranes and organelles.
Instructions for Use:
Add this product to the medium at a dilution ratio of 100×. One bottle can prepare 1 liter of culture medium. Supplement the medium with 2%–4% fetal bovine serum (FBS) at the early stage of culture. The FBS concentration can be lowered further after cell adaptation.
Precautions:
1. This product is sterilized via filtration with a 0.22 μm membrane.
2. Perform strict aseptic operations during use to prevent contamination.
3. This is a 100× concentrated solution. Dilute it as required before application.
4. For research use only. Not for diagnostic or therapeutic purposes.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | I747213 | |
| 分析证书 | I747213 |
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