计算溶液所需的质量、体积或浓度。
甲酵刚果红染色液(0.5%)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本产品为组织病理专用染色试剂，主要用于石蜡切片、冰冻切片中淀粉样物质（如AL型、AA型淀粉样变）的特异性染色，可通过光镜观察淀粉样物质的分布与形态，偏振光下可呈现特征性苹果绿双折光，用于病理诊断及相关实验研究。
使用方法（仅供参考以石蜡切片为例）：
1、脱蜡至水：将石蜡切片依次放入二甲苯Ⅰ（5分钟）→ 二甲苯Ⅱ（5分钟）→ 100%乙醇（2分钟）→ 95%乙醇（2分钟）→ 80%乙醇（2分钟），最后用蒸馏水漂洗2次，每次1分钟。
2、染色处理：将切片放入0.5%甲醇刚果红染液中，室温下染色10-20分钟，染色期间可轻轻晃动染液，确保切片染色均匀。
3、分化处理：取出切片，放入碱性乙醇分化液（0.2%KOH溶于80%乙醇）中快速分化5-10秒，期间需在显微镜下观察，直至背景近无色、淀粉样区域保留红色，立即取出。
4、水洗返蓝：将切片用流水冲洗3-5分钟，彻底去除残留的分化液，避免影响后续染色效果。
5、复染细胞核：将切片放入Mayer苏木素染液中复染1-2分钟，取出后用流水冲洗，直至细胞核呈蓝色。
6、脱水透明：依次放入梯度乙醇（95%乙醇2分钟→100%乙醇2分钟×2次）→ 二甲苯（5分钟×2次），使切片透明。
7、封固观察：用中性树胶滴加在切片上，盖上盖玻片封固，待树胶干燥后，光镜或偏振光显微镜下观察。
染色结果：
光镜下：淀粉样物质呈橘红色，细胞核呈蓝色，背景无色或淡粉色。
偏振光显微镜下：淀粉样物质呈特征性苹果绿双折光，为阳性结果。
注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。
2、本产品仅用于科研，严禁他用。
This product is a special staining reagent for histopathology, mainly used for the specific staining of amyloid substances (such as AL-type and AA-type amyloidosis) in paraffin sections and frozen sections. The distribution and morphology of amyloid substances can be observed under light microscopy, and they exhibit characteristic apple-green birefringence under polarized light, which is used for pathological diagnosis and related experimental research.
Usage (for reference only, taking paraffin sections as an example):
1. Deparaffinization to water: Place the paraffin sections sequentially in Xylene I (5 min) → Xylene II (5 min) → 100% ethanol (2 min) → 95% ethanol (2 min) → 80% ethanol (2 min), and finally rinse with distilled water twice, 1 min each time.
2. Staining: Immerse the sections in 0.5% methanol Congo Red staining solution and stain at room temperature for 10–20 min. Gently agitate the staining solution during the process to ensure uniform staining.
3. Differentiation: Take out the sections and differentiate rapidly in alkaline ethanol differentiation solution (0.2% KOH dissolved in 80% ethanol) for 5–10 seconds. Monitor under a microscope until the background is nearly colorless and the amyloid regions retain red color, then remove immediately.
4. Washing and bluing: Rinse the sections under running water for 3–5 min to completely remove residual differentiation solution and avoid affecting subsequent staining results.
5. Nuclear counterstaining: Counterstain the sections in Mayer's hematoxylin solution for 1–2 min, then rinse under running water until the cell nuclei appear blue.
6. Dehydration and clearing: Place sequentially in graded ethanol (95% ethanol 2 min → 100% ethanol 2 min, twice) → Xylene (5 min, twice) to clear the sections.
7. Mounting and observation: Add neutral balsam onto the sections, cover with a coverslip, and after the balsam dries, observe under light microscopy or polarized light microscopy.
Staining Results:
Light microscopy: Amyloid substances appear orange-red, cell nuclei appear blue, and the background is colorless or pale pink.
Polarized light microscopy: Amyloid substances exhibit characteristic apple-green birefringence, indicating a positive result.
Precautions:
1. For your safety and health, please wear a laboratory coat and disposable gloves during operation.
2. This product is for research use only; strictly prohibited for other purposes.
规格
别名
甲醇刚果红溶液 | 刚果红染液
英文别名
Congo Red methanol solution | Congo Red stain
规格或纯度
BioReagent,生物染色剂,用于显微镜,0.5%
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Methanol Congo Red Staining Solution (0.5%)
储存条件
避光,室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434646 |
分析证书 | M1520403 |
技术文档和文章
溶液计算器
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