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基因编辑
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核酸内切酶 VIII
- 种属: 大肠杆菌(E.coli)
- Accession #: P50465.3
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme required to cleave 1pmol of a 34 mer oligonucleotide duplex containing a single AP site in a total reaction volume of 10μl in 1 hour at 37℃ in 1X Endonuclease VIII Reaction Buffer containing 10pmol of fluorescently labeled oligonucleotide duplex
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pA-Tn5 转座酶
- 种属: 大肠杆菌(E.coli)
- Accession #: Q46731
- 表达系统: E. coli
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5'端 DNA/RNA 腺苷酰化酶
- 种属: 嗜热菌腺苷水解酶基因(Adenosylase gene of thermophilic bacteria)
- Accession #: 8UCH_A,A0A075LQ94
- 表达系统: E. coli
- 生物活性: One unit is the amount of enzyme required to catalyze the transfer of 1pmol of AMP to pDNA substrate in a total reaction volume of 20μl in 15 minutes at 65℃.
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热敏磷酸酶
- 种属: 南极细菌TAB5(Antarctic bacterium TAB5)
- Accession #: 2W5W_B,Q9KWY4
- 表达系统: E. coli
- 生物活性: 1 unit refers to the 5 'concave end produced by restriction endonuclease digestion at 37 ℃ for 30 minutes. The enzyme amount required for dephosphorylation of µ g pUC19 vector DNA. The dephosphorylation standard refers to the self linking reaction Can inhibit>95% of DNA re circularization and detect it by transforming E. coli cells.
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Klenow Fragment, Exo-
- 种属: 大肠杆菌(E.coli)
- Accession #: 2KFZ_A,P00582
- 表达系统: E. coli
- 生物活性: One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37℃.
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大肠杆菌聚合酶 I 大片段
- 种属: 大肠杆菌(E.coli)
- Accession #: 2KFZ_A,P00582
- 表达系统: E. coli
- 生物活性: One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37℃.
