聚乙烯醇抗荧光淬灭封片剂又称聚乙烯醇荧光封片剂 (Elvanol Fluorescence Mounting Medium)，是一种用于减缓荧光淬灭的封固剂，又称荧光信号增强封片剂，类似于SIGMA的含DABCO的聚乙烯醇封片剂 (防褪色)。可以用于减缓各种常见荧光染料的荧光淬灭，较普通抗荧光淬灭封片剂的抗淬灭效果大大增强，一般2-3周后才会有所减弱，该封片剂还有增强罗丹明荧光信号的作用。按每个样本需要50-100μl封片剂计算，5ml可以用于至少50样本的封片。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
组织样本、载玻片、盖玻片、荧光显微镜
操作步骤(仅供参考)：
1、贴壁细胞样本
①染色完成后，吸去染色液。
②完全溶解聚乙烯醇抗荧光淬灭封片剂，滴1滴于载玻片上，盖上贴有细胞的盖玻片，让细胞接触封片液，尽量避免气泡。
③在荧光显微镜下观察或4℃避光保存2-3周。
2、组织切片
①染色完成后，吸去染色液。
②完全溶解聚乙烯醇抗荧光淬灭封片剂，滴1滴于组织切片上，盖上盖玻片，让切片接触封片液，尽量避免气泡。
③在荧光显微镜下观察或4℃避光保存2-3周。
3、其它样品
其它样品参考细胞样品或组织切片进行操作。
注意事项：
1、抗荧光淬灭的概念是相对的，样本染色后最好立即观察，如果没有条件，最好应在当天观察。
2、荧光物质均易发生淬灭，染色后的样品宜4℃避光保存。
3、在使用聚乙烯醇抗荧光淬灭封片剂的情况下，虽然可减缓淬灭，仍需尽量避光。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用，以防影响后续实验效果。
Polyvinyl Alcohol Anti-Fluorescence Quenching Mounting Medium, also known as Polyvinyl Alcohol Fluorescence Mounting Medium, is a mounting agent designed to slow down fluorescence quenching. It is also referred to as Fluorescence Signal-Enhancing Mounting Medium, and is similar to Sigma’s polyvinyl alcohol mounting medium containing DABCO (anti-fading type).
This medium can reduce the quenching effect of various common fluorescent dyes, with its anti-quenching performance significantly better than that of regular anti-quenching mounting media. Generally, the anti-quenching effect only begins to weaken after 2-3 weeks. Additionally, this mounting medium has the function of enhancing the fluorescence signal of rhodamine. Based on a dosage of 50-100 μl per sample, 5 ml of the reagent can be used for mounting at least 50 samples. This reagent is intended for research purposes only and not for clinical diagnosis or other applications.
Materials to Be Prepared by Users
Tissue samples, slides, coverslips, fluorescence microscope
Operating Procedures (For Reference Only)
1. Adherent Cell Samples
① After staining, aspirate the staining solution completely.
② Fully dissolve the Polyvinyl Alcohol Anti-Fluorescence Quenching Mounting Medium, add 1 drop onto the slide, then place the coverslip with adherent cells onto the medium. Ensure the cells are in contact with the mounting medium and minimize bubble formation.
③ Observe under a fluorescence microscope immediately, or store the slides at 4 °C away from light for 2-3 weeks.
2. Tissue Sections
① After staining, aspirate the staining solution completely.
② Fully dissolve the Polyvinyl Alcohol Anti-Fluorescence Quenching Mounting Medium, add 1 drop onto the tissue section, then place a coverslip over it. Ensure the section is in contact with the mounting medium and minimize bubble formation.
③ Observe under a fluorescence microscope immediately, or store the slides at 4 °C away from light for 2-3 weeks.
3. Other Samples
Follow the procedures for adherent cell samples or tissue sections.
Precautions
1. The concept of anti-fluorescence quenching is relative. Stained samples are best observed immediately after staining; if immediate observation is not feasible, observation should be completed on the same day.
2. All fluorescent substances are prone to quenching. Stained samples should be stored at 4 °C away from light.
3. Although this Polyvinyl Alcohol Anti-Fluorescence Quenching Mounting Medium can slow down quenching, samples should still be protected from light as much as possible during use and storage.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
5. Use the reagent as soon as possible after opening to avoid compromising subsequent experimental results.
ZJ26F0535668
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