是否无菌：是
293T（HEK-293T）细胞是基于人胚肾细胞改造的永生化细胞系，具备转染效率高、增殖速度快、可高密度培养的特点，是慢病毒/腺病毒包装、重组蛋白表达、质粒转染、基因功能验证、细胞模型构建的核心工具细胞。该细胞对血清品质极其敏感，普通胎牛血清常存在杂蛋白干扰、内毒素偏高、生长因子配比不均、微量外源污染等问题，易导致细胞贴壁松散、抱团脱落、转染效率下降、病毒滴度偏低、批次实验重复性差等问题。
本品为293T细胞专属定制特级胎牛血清，精选合规牧场6～8月龄健康胎牛血源，采用专属精细化精制工艺，优化生长因子、黏附因子配比，严控内毒素、溶血杂质、外源污染及蛋白沉淀，最大程度适配293T细胞贴壁生长、高密度扩增、瞬时转染与病毒包装的核心需求。
产品优势
1.专属细胞适配性：优化促贴壁、促增殖活性组分，解决293T细胞易飘壁、长势弱、传代退化快等问题，细胞形态规整、折光性好。
2.高转染高滴度适配：低内毒素、低杂蛋白背景，大幅降低细胞应激反应，显著提升质粒转染效率、慢病毒/腺病毒包装滴度，保障蛋白表达产量稳定。
3.极低杂质干扰：多级除溶血、除脂蛋白、除微颗粒工艺，冻融后无大量絮状沉淀，不污染培养基、不影响细胞观察与后续实验。
4.高安全无外源污染：严格病毒、支原体、细菌、真菌筛查，无噬菌体及隐性外源污染，适配病毒学实验与基因工程研究。
5.批次高度稳定：每批次均经过293T细胞功能验证，严控增殖速率、转染效率、细胞活率，彻底规避普通血清批次差异导致的实验失败。
生产工艺简介
本品采用源头优选+低温精制+多级除杂+纳米除菌+细胞专属验证标准化生产工艺，全程C级洁净区、A级无菌灌装，核心工序如下：
1.源头血源筛选：选用非疫区健康胎牛无菌采血，低温密闭冷链转运，严控溶血度，剔除高杂质、高内毒素不合格原料，从源头保障血清品质。
2.低温梯度离心精制：多级低温高速离心，彻底去除血细胞碎片、纤维蛋白、脂蛋白沉淀、脂质微颗粒，降低血清杂蛋白本底，减少293T细胞培养杂质干扰。
3.活性组分保留工艺：全程低温处理，不高温灭活、不化学改性，完整保留EGF、FGF、黏附蛋白、生长激素等核心活性组分，保障细胞快速贴壁与增殖。
4.多级纳米无菌过滤：采用0.1μm终端过滤，逐级截留细菌、真菌、支原体、微颗粒，实现无菌高纯品质。
5.专属功能验证放行：所有成品均需通过293T细胞连续传代培养、增殖速率、转染效率、病毒包装功能性测试，合格后方可批次放行。
适用范围
专用于HEK-293T细胞体外贴壁/悬浮培养、连续传代扩增、瞬时转染实验、慢病毒/腺病毒/AAV病毒包装、重组蛋白表达、基因沉默/过表达功能验证、药物筛选细胞模型构建；同时适配293、CHO、Vero等常规工程细胞系培养。
使用方法
1.融化方法：-20°C冻存血清优先置于4°C冰箱过夜解冻，期间轻轻颠倒混匀；完全融化后37°C水浴复温，避免室温久置、避免反复冻融。
2.培养基配制：推荐配比：高糖DMEM培养基 + 10% 本品胎牛血清 + 1% 双抗（可选），充分混匀后即可用于293T细胞培养。
3.培养条件：37°C、5% CO2、饱和湿度培养箱常规培养，按需传代换液。
4.注意事项：融化后少量絮状沉淀为正常蛋白析出，不影响使用；无需自行过滤，避免损耗活性组分。
注意事项
1.本品仅用于体外细胞科研实验，禁止用于人体、临床及活体注射使用。
2.血清融化后出现微量白色絮状沉淀为天然蛋白析出，属于正常现象，不会影响细胞生长与实验结果。
3.避免与普通低等级血清混用，防止引入杂质、内毒素及外源污染，影响293T细胞转染与病毒实验效果。
4.操作全程无菌环境，避免人为微生物污染，开封后尽快使用。
5.不同批次血清无需驯化，本品批次稳定性高，可直接替换使用。
免责声明
本产品仅用于科研或工业用途，不适用于临床治疗。
用户需根据自身实验体系验证血清适用性，生产方不承担因操作不当导致的实验结果偏差责任。
293T (HEK-293T) cells are an immortalized cell line derived from modified human embryonic kidney cells. They are characterized by high transfection efficiency, rapid proliferation, and the ability to be cultured at high density, making them a core tool cell line for lentiviral/adenoviral packaging, recombinant protein expression, plasmid transfection, gene function verification, and cell model construction. These cells are extremely sensitive to serum quality. Common fetal bovine serum often has issues such as interference from miscellaneous proteins, high endotoxin levels, imbalanced growth factor ratios, and trace exogenous contamination, which can easily lead to loose adherence, clumping and detachment, decreased transfection efficiency, low viral titers, and poor batch-to-batch experimental reproducibility.
This product is a premium fetal bovine serum exclusively customized for 293T cells. It is sourced from healthy fetal cattle aged 6–8 months from compliant pastures, and produced using a dedicated, refined manufacturing process that optimizes the ratios of growth factors and adhesion factors, while strictly controlling endotoxins, hemolytic impurities, exogenous contaminants, and protein precipitates. It is maximally adapted to meet the core requirements of 293T cell adherence, high-density expansion, transient transfection, and viral packaging.
Product Advantages
1.Cell-specific adaptability: Optimized pro-adhesion and pro-proliferation active components address issues such as easy detachment, weak growth, and rapid passage-induced senescence in 293T cells, resulting in regular cell morphology and good refractility.
2.High transfection and high titer compatibility: Low endotoxin and low background of miscellaneous proteins significantly reduce cellular stress responses, markedly improve plasmid transfection efficiency and lentiviral/adenoviral packaging titers, and ensure stable protein expression yields.
3.Extremely low impurity interference: Multi-stage hemolysis removal, lipoprotein removal, and microparticle removal processes result in no significant flocculent precipitates after freeze-thaw cycles, avoiding contamination of culture media and interference with cell observation and subsequent experiments.
4.High safety and freedom from exogenous contamination: Rigorous screening for viruses, mycoplasma, bacteria, and fungi, with no bacteriophages or hidden exogenous contaminants, making it suitable for virology research and genetic engineering studies.
5.High batch-to-batch consistency: Each batch is functionally validated with 293T cells, with strict control over proliferation rate, transfection efficiency, and cell viability, completely avoiding experimental failures caused by batch-to-batch variations common in regular sera.
Production Process Overview
1.This product is manufactured using a standardized process that integrates source selection, low-temperature refining, multi-stage impurity removal, nano-sterilization, and cell-specific validation. The entire process is carried out in Grade C clean areas with Grade A aseptic filling. The core steps are as follows:
2.Raw material screening: Aseptic blood collection from healthy cattle in non-epidemic regions, transported via low-temperature closed cold chain with strict control over hemolysis levels. Raw materials with high impurities or high endotoxin are rejected to ensure serum quality from the source.
3.Low-temperature gradient centrifugation refining: Multi-stage low-temperature high-speed centrifugation thoroughly removes blood cell debris, fibrin, lipoprotein precipitates, and lipid microparticles, reducing the background of miscellaneous serum proteins and minimizing impurity interference in 293T cell culture.
4.Active component preservation: The entire process is carried out at low temperature without heat inactivation or chemical modification, fully preserving core active components such as EGF, FGF, adhesion proteins, and growth hormones to support rapid cell adherence and proliferation.
5.Multi-stage nano-sterile filtration: Terminal filtration with 0.1 μm pore size sequentially retains bacteria, fungi, mycoplasma, and microparticles, achieving sterile, high-purity quality.
6.Cell-specific functional validation and release: All finished products must pass functional tests on 293T cells, including serial passage culture, proliferation rate, transfection efficiency, and viral packaging performance, before batch release.
Scope of Application
Specifically designed for HEK-293T cell in vitro adherent/suspension culture, serial passage expansion, transient transfection experiments, lentiviral/adenoviral/AAV packaging, recombinant protein expression, gene silencing/overexpression functional validation, and drug screening cell model construction. It is also compatible with routine engineering cell lines such as 293, CHO, and Vero.
Usage Instructions
1.Thawing method: Serum stored at –20°C should preferably be thawed overnight at 4°C in a refrigerator, with gentle inversion mixing during the process. After complete thawing, warm in a 37°C water bath. Avoid prolonged exposure at room temperature and avoid repeated freeze-thaw cycles.
2.Medium preparation: Recommended formulation: High-glucose DMEM medium + 10% of this serum + 1% antibiotics (optional). Mix thoroughly before use for 293T cell culture.
3.Culture conditions: Routine culture in a 37°C, 5% CO₂, humidified incubator, with medium changes and passaging as needed.
Note: A small amount of flocculent precipitate after thawing is normal protein precipitation and does not affect use. Do not filter the serum yourself, as this may deplete active components.
Precautions
1.This product is for in vitro cell research use only. It is prohibited for human, clinical, or in vivo injection applications.
2.Trace white flocculent precipitate after thawing is natural protein precipitation and is a normal phenomenon that does not affect cell growth or experimental results.
3.Avoid mixing with low-grade conventional sera to prevent the introduction of impurities, endotoxins, and exogenous contaminants that could affect 293T cell transfection and viral experiments.
4.Maintain a sterile environment throughout all operations to prevent microbial contamination. Use promptly after opening.
5.No acclimation is required when switching between batches; this product offers high batch stability and can be directly substituted.
Disclaimer
This product is for research or industrial use only and is not suitable for clinical therapy. Users should validate the suitability of the serum for their own experimental systems. The manufacturer assumes no liability for deviations in experimental results caused by improper handling
ZJ26F0938974
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