是否无菌：是
人二倍体细胞为有限传代的正常体细胞，具备遗传背景稳定、无致瘤性、安全性高的特点，是病毒性疫苗制备、病毒分离鉴定、细胞衰老机制研究、生物制品研发的核心细胞模型。二倍体细胞对培养环境极其敏感，存在传代寿命有限、增殖速率慢、极易老化凋亡、对杂质与外源污染耐受度极低的特性。普通胎牛血清存在外源病毒风险高、内毒素波动大、杂蛋白与微颗粒杂质多、生长因子配比失衡等问题，极易导致二倍体细胞形态异常、提前衰老、增殖停滞、传代寿命缩短，严重影响疫苗生产稳定性与科研实验重复性。
本品为二倍体细胞专属定制特级胎牛血清，针对人二倍体细胞 (2BS、MRC-5、WI-38 等) 的生长特性与生物制品级培养要求定向优化。
甄选高标准非疫区特级胎牛血源，通过低温精细化精制、多重病原严控、低应激组分优化、活性因子稳态保留等专属工艺，最大限度去除各类干扰杂质，精准匹配二倍体细胞慢增殖、低应激、长效传代的培养需求。
本品纯度高、安全性优、批次稳定性极强，可有效延缓细胞老化、维持细胞形态稳定与传代能力，是二倍体细胞科研培养、规模化扩增、疫苗研发生产的专用高端原料。
产品优势
1. 延缓细胞衰老，延长传代寿命：精准配比抗氧化、促增殖活性因子，有效降低细胞氧化应激损伤，减少空泡、老化、脱落现象，稳定二倍体细胞正常传代周期，避免提前衰亡。
2. 超高生物安全等级：多重牛源病毒、支原体、微生物专项筛查，严控外源病原污染风险。
3. 低应激高纯培养体系：超低内毒素、低溶血、低杂蛋白本底，无多余脂蛋白与微颗粒干扰，培养体系洁净稳定，杜绝细胞应激性凋亡与增殖抑制。
4. 细胞形态高度稳定：优化黏附因子与营养配比，保障二倍体细胞贴壁紧实、形态均一、胞体透亮，长期传代无形态畸变，适配长期连续培养实验。
5. 生物制品级批次稳定性：每批次均经过主流二倍体细胞功能验证，严控细胞增殖速率、活率、传代稳定性，批次差异极小，满足科研及规模化生产需求。
生产工艺简介
本品采用高标准血源甄选、低温锁活精制、多重除杂纯化、全维度病原筛查、纳米无菌过滤、二倍体细胞专属功能验证全套生物制品级工艺，C 级洁净区生产、A 级无菌灌装，核心工序如下：
1. 高端专属血源筛选：严格甄选无疫区、无疫病健康胎牛，无菌密闭心脏穿刺采血，全程低温冷链锁活，筛选低溶血、低内毒素、生长因子活性高的优质原料，从源头杜绝病原与杂质隐患。
2. 低温多级精制除杂：全程 4℃低温环境完成凝血分离、梯度高速离心，彻底去除血细胞碎片、纤维蛋白沉淀、脂蛋白微粒及变性杂蛋白，大幅降低培养体系杂质背景，适配敏感二倍体细胞培养。
3. 活性组分稳态保留工艺：全程无高温、无化学改性处理，完整保留 EGF、FGF、抗氧化因子、黏附蛋白等核心活性组分，精准支撑二倍体细胞缓慢、稳定、长效增殖。
4. 多重纳米无菌过滤：采用 0.1μm 终端串联过滤工艺，逐级截留细菌、真菌、支原体、微量微粒，实现高纯无菌标准。
5. 二倍体细胞专属放行验证：每批次成品均通过 MRC-5、2BS 主流二倍体细胞连续传代测试，验证细胞活率、形态稳定性、传代周期及抗老化能力，全部达标后方可批次放行。
二倍体细胞功能指标
细胞贴壁性能：二倍体细胞贴壁快速、牢固，无漂浮、脱落现象
细胞活率：连续传代 5 代以上细胞活率≥93%，无大面积老化凋亡
形态稳定性：细胞形态规整、胞体均匀、折光性好，无空泡、畸变、纤维化异常
传代稳定性：可稳定维持二倍体细胞正常传代周期，无提前衰老、增殖停滞问题
病毒适配性：支持病毒稳定侵染、增殖与收获，适配病毒分离与疫苗制备体系。
适用范围
专用于人二倍体细胞专属培养体系，核心适用场景：2BS、MRC-5、WI-38 等所有人二倍体细胞复苏、传代、保种与规模化扩增、人类病毒分离培养、病毒性疫苗研发与制备、细胞衰老机制研究、低应激细胞模型构建、长期连续细胞培养实验。
不适用场景：去外泌体高精度组学实验、昆虫细胞培养、超高密度快速增殖细胞体系。
使用方法
1. 血清融化：将冻存血清置于 4℃冰箱缓慢过夜解冻，期间轻柔颠倒混匀，保证组分均匀；完全解冻后 37℃水浴复温备用，严禁快速高温解冻与反复冻融。
2. 培养基配制：推荐配比：MEM / 高糖 DMEM 基础培养基 + 10% 本品胎牛血清，可根据实验需求选择性添加 1% 青霉素 - 链霉素双抗。
3. 常规培养操作：严格按照二倍体细胞培养规程传代、换液，维持稳定营养环境，减少细胞应激刺激。
培养条件：37℃、5% CO₂ 、饱和湿度恒温培养箱常规培养，根据细胞生长状态及时换液传代。
注意事项
1. 血清融化后出现微量细小蛋白析出为正常现象，不影响二倍体细胞生长、传代及实验效果，无需过滤处理。
2. 二倍体细胞为高敏感有限传代细胞，实验全程建议固定使用本品，切勿随意更换普通血清，避免造成细胞应激、老化、传代异常。
3. 严格遵守无菌操作规范，开封后尽快使用，防止人为微生物污染影响细胞培养稳定性。若血清出现浑浊、结块、变色、异味等异常情况，禁止使用。
免责声明
本产品仅用于科研或工业用途，不适用于临床治疗。
用户需根据自身实验体系验证血清适用性，生产方不承担因操作不当导致的实验结果偏差责任。
Human diploid cells are normal somatic cells with limited passage capacity. Featuring stable genetic background, non-tumorigenicity and high biosafety, they serve as core cell models for viral vaccine production, virus isolation and identification, research on cellular senescence mechanisms, and biological product development.
Diploid cells are extremely sensitive to culture environments, characterized by limited passage lifespan, slow proliferation rate, high susceptibility to senescence and apoptosis, and extremely low tolerance to impurities and exogenous contaminants. Conventional fetal bovine serum (FBS) carries risks such as high exogenous virus load, fluctuating endotoxin levels, abundant hybrid proteins and particulate impurities, and unbalanced growth factor ratios. These defects readily trigger abnormal cell morphology, premature senescence, proliferation arrest and shortened passage lifespan of diploid cells, severely compromising the stability of vaccine manufacturing and reproducibility of research experiments.
This premium-grade FBS is custom-formulated exclusively for diploid cells, optimized to match the growth characteristics of human diploid cells (2BS, MRC-5, WI-38, etc.) and culture standards for biological products.
We source premium fetal bovine blood from non-epidemic regions. Through proprietary processes including low-temperature fine purification, stringent multi-pathogen control, low-stress component optimization and steady retention of active factors, most interfering impurities are eliminated to precisely satisfy the culture demands of diploid cells for slow proliferation, low stress and long-term passaging.
Boasting high purity, superior biosafety and exceptional batch-to-batch consistency, this serum effectively delays cellular senescence while maintaining stable cell morphology and passaging capacity. It is a premium dedicated raw material for research culture, large-scale expansion, vaccine R&D and production of diploid cells.
Product Advantages
1. Delays cellular senescence and extends passage lifespan: Precisely balanced antioxidant and pro-proliferation active factors mitigate oxidative stress damage to cells, reducing vacuolation, senescence and cell detachment. It stabilizes the normal passage cycle of diploid cells and prevents premature cell death.
2. Ultra-high biosafety level: Specialized multi-assay screening for bovine-derived viruses, mycoplasma and microorganisms to rigorously control risks of exogenous pathogenic contamination.
3. Low-stress high-purity culture system: Ultra-low endotoxin, hemolysis and background hybrid proteins, free of redundant lipoproteins and micro-particle interference. The clean and stable culture system eliminates stress-induced apoptosis and proliferation inhibition of cells.
4. Highly stable cell morphology: Optimized adhesion factors and nutrient ratios ensure tight attachment, uniform shape and transparent cytoplasm of diploid cells. No morphological distortion occurs after long-term serial passaging, making it suitable for long-term continuous culture experiments.
5. Batch consistency compliant with biological product standards: Each batch undergoes functional validation on mainstream diploid cell lines. Strict control over cell proliferation rate, viability and passage stability results in minimal batch variation, meeting demands of laboratory research and large-scale manufacturing.
Brief Introduction to Production Processes
The serum is manufactured with a full set of biological-product-grade workflows: high-standard blood source selection, low-temperature activity-locked refining, multi-stage impurity removal and purification, comprehensive pathogen screening, nano sterile filtration and exclusive functional verification for diploid cells. Production is carried out in Grade C cleanrooms with Grade A aseptic filling. Key procedures are listed below:
1. Premium exclusive blood source screening: Healthy pathogen-free fetal cattle from non-epidemic zones are selected. Blood is collected via sterile closed cardiac puncture under full low-temperature cold chain to preserve bioactivity. Only high-quality raw materials with low hemolysis, low endotoxin and highly active growth factors are adopted to eliminate hidden risks of pathogens and impurities at the source.
2. Multi-stage low-temperature purification for impurity removal: Coagulation separation and gradient high-speed centrifugation are performed entirely at 4 °C to thoroughly remove blood cell debris, fibrin precipitates, lipoprotein microparticles and denatured hybrid proteins. This drastically cuts background impurities in the culture system for culturing sensitive diploid cells.
3. Stable retention of active components: No high-temperature treatment or chemical modification is applied throughout the process, fully preserving core active components such as EGF, FGF, antioxidants and adhesion proteins to support steady, slow and long-term proliferation of diploid cells.
4. Multi-stage nano sterile filtration: A terminal tandem filtration system with 0.1 μm filters sequentially intercepts bacteria, fungi, mycoplasma and trace particulates to meet high-purity sterility criteria.
5. Exclusive batch release verification for diploid cells: Every finished batch undergoes serial passaging tests on mainstream diploid cell lines (MRC-5, 2BS). Cell viability, morphological stability, passage cycle and anti-senescence performance are verified, and batches are released only after all indicators pass inspection.
Functional Indicators for Diploid Cell Culture
- Cell adhesion performance: Diploid cells attach rapidly and firmly without floating or detachment.
- Cell viability: Viability remains ≥93% after more than 5 consecutive passages, with no large-scale senescence or apoptosis.
- Morphological stability: Regular, evenly sized cells with good refractivity; no vacuoles, malformations or fibrotic abnormalities.
- Passage stability: Sustains the normal passage cycle of diploid cells without premature senescence or proliferation arrest.
- Virus compatibility: Supports stable virus infection, propagation and harvest, compatible with workflows for virus isolation and vaccine preparation.
Scope of Application
Exclusively designed for culture systems of human diploid cells. Core applicable scenarios:
Resuscitation, passaging, seed preservation and large-scale expansion of all human diploid cell lines including 2BS, MRC-5 and WI-38; isolation and culture of human viruses; R&D and manufacturing of viral vaccines; research on cellular senescence mechanisms; construction of low-stress cell models; long-term continuous cell culture experiments.
Inapplicable scenarios: High-precision omics experiments requiring exosome depletion, insect cell culture, and cell systems with ultra-high-density rapid proliferation.
Instructions for Use
1. Serum thawing: Place frozen serum in a 4 °C refrigerator for slow overnight thawing; gently invert and mix during thawing to ensure homogeneous components. After complete thawing, warm in a 37 °C water bath before use. Rapid high-temperature thawing and repeated freeze-thaw cycles are strictly prohibited.
2. Medium preparation: Recommended formula: MEM / high-glucose DMEM basal medium supplemented with 10% of this serum. 1% penicillin-streptomycin double antibiotic can be added optionally based on experimental requirements.
3. Routine culture operation: Perform passaging and medium exchange strictly following standard protocols for diploid cells to maintain a stable nutritional environment and reduce cellular stress stimulation.
Culture conditions: Incubate at 37 °C with 5% CO₂ and saturated humidity in a constant-temperature incubator; replace medium and passage cells timely according to cell growth status.
Precautions
1. Trace tiny protein precipitates appearing after serum thawing are normal and will not affect the growth, passaging or experimental results of diploid cells; filtration treatment is unnecessary.
2. Diploid cells are highly sensitive cells with limited passage capacity. Consistent use of this serum throughout experiments is recommended. Arbitrary switching to ordinary FBS will induce cellular stress, senescence and abnormal passaging.
3. Strictly follow aseptic operation standards and use the serum promptly after opening to avoid artificial microbial contamination that destabilizes cell culture. Discontinue use if the serum turns turbid, clumpy, discolored or develops abnormal odor.
Disclaimer
This product is for research or industrial use only and shall not be applied to clinical treatment.
Users shall verify the compatibility of the serum with their own experimental systems. The manufacturer bears no liability for deviations in experimental results caused by improper operation.
ZJ26F0938978
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