α-Glucosidase, a carbohydrate hydrolyzing enzyme, catalyzes the liberation of α-glucose from the non-reducing end of the substrate. α-Glucosidase can facilitate the absorption of glucose by the small intestine. Inhibition of α-Glucosidase is an effective management of non-insulin-dependent diabetes mellitus (NIDDM).
Reaction
Product description
Appearance:
White amorphous powder
Source:
Microorganism
Enzyme Commission Number:
EC 3.2.1.20
CAS Number:
9001-42-7
Storage temperature:
-20℃
Activity:
≥15U/mg enzyme powder; ≥100U/mg protein
Unit definition:
One unit will convert one micromole of PNPG to PNP per min at pH 7.0 at 37℃.
Properties
Stability:
Stable at -20℃ for at least two years
Molecular weight:
65kD (SDS-PAGE)
Isoelectric point:
6.0
Michaelis constant:
1.4×10⁻³M (PNPG)
Optimum pH:
6.0-6.5
{Fig. 1}
Optimum temperature:
60℃
{Fig. 3}
pH Stability:
6.0-8.5 (25℃, 24hr)
{Fig. 2}
Thermal stability:
< 60℃ (pH 7.0, 30min)
{Fig. 4}
Effect of various chemicals:
Cu²⁺, Fe³⁺, Ni²⁺, SDS
{Table 1}
Table 1. Effect of Various Chemicals on AGH
[The enzyme dissolved in 20mM Tris-HCl buffer, pH 7.0 (5U/ml) was incubated with each chemical at 37 for 2hr.]