计算溶液所需的质量、体积或浓度。
Recombinant Human MMP9 Protein (rp148898) - Protein Bioactivity
Measured in a cell migration assay using A549 cells. 1.0 ng/mL of Recombinant Human MMP-9 (rp148898) can effectively induce A549 cells migration.
ActiBioPure™,His标签,无动物源,无叠氮钠,无载体,生物活性,高性能 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Purity
>95% SDS-PAGE.
Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Background
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. In addition to the human enzyme, the recombinant mouse MMP-9 is also available.
Recombinant Human MMP9 Protein (rp148898) - Protein Bioactivity
Measured in a cell migration assay using A549 cells. 1.0 ng/mL of Recombinant Human MMP-9 (rp148898) can effectively induce A549 cells migration.
Recombinant Human MMP9 Protein (rp148898) - SDS-PAGE
3 μg/lane of Recombinant Human MMP9 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 93.7 kDa under reducing conditions and 250.0 & 89.5 kDa under non-reducing conditions.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp148898 | |
| 分析证书 | rp148898 | |
| 分析证书 | rp148898 | |
| 分析证书 | rp148898 | |
| 分析证书 | rp148898 | |
| 分析证书 | rp148898 | |
| 分析证书 | rp148898 | |
| 分析证书 | rp148898 | |
| 分析证书 | rp148898 |
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