1. Measured in a cell migration assay using A549 cells. 5.0 ng/mL of Recombinant Human MMP-9 can effectively induce A549 cells migration. 2. Immobilized Recombinant Human MMP-9 Protein (rp181214) at 1.0 μg/mL can bind Andecaliximab (anti-MMP9), the EC50 for this effect is 12.14 ng/mL.
Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description: Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.
规格
产品名称
Recombinant Human MMP-9 Protein
别名
重组人MMP-9蛋白 | 重组人MMP-9蛋白
英文别名
matrix metalloproteinase-9 | MMP9 | MMP-9 | type V collagenase | 92 kDa gelatinase | 92 kDa type IV collagenase | CLG4B | EC 3.4.24 | EC 3.4.24.35 | Gelatinase B | GELB | macrophage gelatinase | MANDP2 | matrix metallopeptidase 9 | matrix metalloproteinas
1. Measured in a cell migration assay using A549 cells. 5.0 ng/mL of Recombinant Human MMP-9 can effectively induce A549 cells migration. 2. Immobilized Recombinant Human MMP-9 Protein (rp181214) at 1.0 μg/mL can bind Andecaliximab (anti-MMP9), the EC50 for this effect is 12.14 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human MMP-9 Protein (rp181214) - ELISA Immobilized Recombinant Human MMP-9 Protein (rp181214) at 1.0 μg/mL can bind Andecaliximab (anti-MMP9), the EC50 for this effect is 12.14 ng/mL.
Recombinant Human MMP-9 Protein (rp181214) - SDS-PAGE 2 μg/lane of Recombinant Human MMP-9 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 81.8-106.3 kDa under reducing conditions.