级别和纯度: 无动物源?无动物源（Animal Free）—— 不含动物源成分生产，降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。高性能?高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥95%(SDS-PAGE)expressed in HEK293; 见COA
Accession #
P17948
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 26.0 ng/mL in the presence of 10 ng/mL rhVEGF165 (rp156339).
无动物源,无载体,生物活性,ActiBioPure™,高性能,His标签,≥95%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
Vascular endothelial growth factor receptor 1, also known as VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor and FLT1, is a single-pass type I membrane protein and secreted protein which belongs to theprotein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 / FLT1 contains sevenIg-like C2-type (immunoglobulin-like) domains and oneprotein kinase domain. VEGFR-1 / FLT1 is expressed mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR-1 / FLT1 is not expressed in tumor cell lines. VEGFR-1 / FLT1 is an essential receptor tyrosine kinase that regulates mammalian vascular development and embryogenesis. EGF-induced angiogenesis requires inverse regulation of VEGFR-1 and VEGFR-2 in tumor-associated endothelial cells. VEGFR-1 / FLT1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.
Post-translational: N-glycosylated. Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-1169 is important for interaction with PLCG. Phosphorylation at Tyr-1213 is important for interaction with PIK3R1, PTPN11, GRB2, and PLCG. Phosphorylation at Tyr-1333 is important for endocytosis and for interaction with CBL, NCK1 and CRK. Is probably dephosphorylated by PTPRB.
生物活性
Measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 26.0 ng/mL in the presence of 10 ng/mL rhVEGF165 (rp156339).
109.5-130.6 kDa, under reducing conditions; 105.3-126.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human VEGFR1/Flt-1 Protein (rp181241) - Protein Bioactivity
Measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 26.0 ng/mL in the presence of 10 ng/mL rhVEGF165 (rp156339).
Recombinant Human VEGFR1/Flt-1 Protein (rp181241) - SDS-PAGE
3 μg/lane of Recombinant Human VEGFR1/Flt-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 109.5-130.6 kDa under reducing conditions and 105.3-126.2 kDa under non-reducing conditions.
ZJ26F0535865
危险品化学品经营许可证（带存储）