ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.
Post-translational: Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204. Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination; leading to activation. Deubiquitinated by CYLD.
生物活性
Immobilized Recombinant Human ERK1 Protein (rp184918) at 1.0 μg/mL can bind Recombinant ERK1 Antibody (Ab102223) with the EC50 of 28.7 ng/mL.
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
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Recombinant Human ERK1 Protein (rp184918) - ELISA Immobilized Recombinant Human ERK1 Protein (rp184918) at 1.0 μg/mL can bind Recombinant ERK1 Antibody (Ab102223) with the EC₅₀ of 28.7 ng/mL.
Recombinant Human ERK1 Protein (rp184918) - SDS-PAGE 3 μg/lane of Recombinant Human ERK1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 42.2 kDa.