Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.38 ng/mL. Immobilized Recombinant Mouse IL-15R alpha Protein (rp168266) at 2.0 μg/mL can bind Recombinant Human IL-15 Protein (rp215091) with the EC50 of 115.0 ng/mL.
The protein encoded by the IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietic receptor subunits and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported.
生物活性
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.38 ng/mL. Immobilized Recombinant Mouse IL-15R alpha Protein (rp168266) at 2.0 μg/mL can bind Recombinant Human IL-15 Protein (rp215091) with the EC50 of 115.0 ng/mL.
80.0-92.0 kDa, under reducing conditions; 68.0-80.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human IL-15 Protein (rp215091) - Protein Bioactivity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀for this effect is typically 0.38 ng/mL.
Recombinant Human IL-15 Protein (rp215091) - Binding Activity Immobilized Recombinant Mouse IL-15R alpha Protein (rp168266) at 2.0 μg/mL can bind Recombinant Human IL-15 Protein (rp215091) with the EC₅₀ of 115.0 ng/mL.
Recombinant Human IL-15 Protein (rp215091) - SDS-PAGE 3 μg/lane of Recombinant Human IL-15 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 80.0-92.0 kDa under reducing conditions and 68.0-80.0 kDa under non-reducing conditions. The protein as both dimers and monomers under non-reducing (NR) conditions suggests that a portion of the protein forms dimers. This dimerization may be mediated by (or stabilized by) disulfide bonds (Uniprot:P40933).