计算溶液所需的质量、体积或浓度。
| 活性类型 | 活性值-log(M) | 作用机制 | 期刊 | 参考文献(PubMed IDs) |
|---|
有货
为什么选择此级别
Moligand™ 级 提供 ≥99% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,充氩。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 34 篇同行评审文献引用。
概述
易溶于水、甲醇、热乙醇、热丙醇、热丙酮、热乙酸乙酯、吡啶、冰醋酸中，少量溶于热的氯仿中。水中溶解度:46 g/L (20°C)。 Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine. It is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.主要用于抗艾滋病药物（如齐多呋啶、石达呋啶）的制备 Thymidine (dT) is a pyrimidine deoxyriboside (deoxynucleoside) that upon sequential phosphorylation to deoxythymidine triphospate (dTTP) is used to synthesize DNA and the T-loop of transfer RNA. Thymidine is as substrate of Thymidine kinase EC 2.7.1.21 which phosphorylates the nucleoside with a phosphate derived from ATP. dT may be used to study the distribution, specificity and kinetics of a family of thymidine kinase(s). Thymidine has been used: · as a supplement in HL5-modified medium for cell culture · to incubate cells in cell-cycle analysis · in cell synchronization
Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine. It is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.
Thymidine (dT) is a pyrimidine deoxyriboside (deoxynucleoside) that upon sequential phosphorylation to deoxythymidine triphospate (dTTP) is used to synthesize DNA and the T-loop of transfer RNA. Thymidine is as substrate of Thymidine kinase EC 2.7.1.21 which phosphorylates the nucleoside with a phosphate derived from ATP. dT may be used to study the distribution, specificity and kinetics of a family of thymidine kinase(s).
规格
别名
β-胸腺嘧啶核苷 | 胸苷 | 1-(2-脱氧-β-D-呋喃核糖)-5-甲基尿嘧啶 | 1-(2-脱氧-β-D-呋喃核糖)胸苷 | 2'-脱氧胸苷 | 胸腺嘧啶核苷
英文别名
EINECS 200-070-4 | DB04485 | 1-((2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydro-furan-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione | 1-((2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione | SR-01000883981 | EN300-
规格或纯度
Moligand™, ≥99%
英文名称
Thymidine
生化机理
嘧啶脱氧核苷。强大的胸苷激酶底物（IC 50 = 16 nM）。与双链DNA中的脱氧腺苷配对。在体内同步S期的细胞。
储存条件
2-8°C储存,充氩
运输条件
低温运输
作用类型
抑制剂
备注
如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。
纯度
≥99%
名称和识别符
PubChem SID
EC号
200-070-4
分子类型
小分子
IUPAC Name
1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
INCHI
1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1
InChi Key
IQFYYKKMVGJFEH-XLPZGREQSA-N
Smiles
CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O
Isomeric SMILES
CC1=CN(C(=O)NC1=O)[C@H]2C[C@@H]([C@H](O2)CO)O
分子量
242.23
Beilstein号
89285
Reaxy-Rn
889638
Reaxys-RN link address
https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=889638&ln=
技术文档
高级数据
系统学分类
分类树（Taxonomy Tree）
-
界（kingdom)
有机化合物
-
超类（Superclass)
核苷、核苷酸及其类似物
-
类（Class)
嘧啶核苷
- 亚类（Subclass） 嘧啶2'-脱氧核糖核苷
-
类（Class)
嘧啶核苷
-
超类（Superclass)
核苷、核苷酸及其类似物
| 界（kingdom) | 有机化合物 |
|---|---|
| 超类（Superclass) | 核苷、核苷酸及其类似物 |
| 类（Class) | 嘧啶核苷 |
| 亚类（Subclass） | 嘧啶2'-脱氧核糖核苷 |
| 中间层级节点（Intermediate Tree Nodes） | 暂无 |
| 直接上位类(Direct Parent) | 嘧啶2'-脱氧核糖核苷 |
| 其他上位类（Alternative Parents） | 嘧啶酮类 羟基嘧啶 氢化嘧啶 四氢呋喃 杂芳族化合物 仲醇 氧环化合物 氮杂环化合物 伯醇 有机光子化合物 有机氮化合物 有机氧化物 碳氢化合物衍生物 |
| 分子骨架（Molecular Framework) | 芳香族杂单环化合物 |
| 取代基（Substituents） | 羟基苯甲酸酯 - 芳香族杂单环化合物 - 氮杂环 - 杂芳香族化合物 - 烃衍生物 - 羟吡啶 - 羟基嘧啶 - 有机硝基化合物 - 有机氧化物 - 有机氧化合物 - 有机杂环化合物 - 有机氮化合物 - 有机氧化合物 - 有机氮化合物 - 氧杂环 - 初级醇 - 嘧啶 - 嘧啶-2'-脱氧核糖核苷 - 嘧啶酮 - 醛基 - 四氢呋喃 |
| 描述（Description） | 该化合物属于嘧啶2'-脱氧核糖核苷类有机化合物。这类化合物由嘧啶与核糖连接而成，其核糖分子2位缺乏羟基。 |
| 外部描述符（External Descriptors） | Deoxyribonucleosides |
三维结构
交互式化学结构模型
关联靶点（人）
TK1
Tchem
胸苷激酶，细胞质(Thymidine kinase, cytosolic)
(2 活性数据)
TYMS
Tclin
Thymidylate synthase
(1651 活性数据)
TK1
Tchem
Thymidine kinase, cytosolic
(627 活性数据)
143B
(353 活性数据)
CCRF-CEM
(65223 活性数据)
CFPAC-1
(421 活性数据)
HL-60
(67320 活性数据)
MT4
(17854 活性数据)
ATH-8 cell line
(300 活性数据)
HEL
(6614 活性数据)
HepG2
(196354 活性数据)
HeLa
(62764 活性数据)
SLC22A1
Tchem
Solute carrier family 22 member 1
(646 活性数据)
APEX1
Tchem
DNA-(apurinic or apyrimidinic site) lyase
(38016 活性数据)
PMP22
Tbio
Peripheral myelin protein 22
(699 活性数据)
ATAD2
Tchem
ATPase family AAA domain-containing protein 2
(598 活性数据)
DCLRE1A
Tbio
DNA cross-link repair 1A protein
(19 活性数据)
关联靶点（其它种属）
TK
Thymidine kinase (276 活性数据)
tmk
Thymidylate kinase (360 活性数据)
dnk
Deoxynucleoside kinase (95 活性数据)
deoA
Thymidine phosphorylase (531 活性数据)
Enterococcus faecium (13803 活性数据)
Mycobacterium tuberculosis (203094 活性数据)
Human alphaherpesvirus 2 (4932 活性数据)
Artemia (698 活性数据)
Mus musculus (284745 活性数据)
Human alphaherpesvirus 1 (11089 活性数据)
Hepatitis B virus (7925 活性数据)
Human immunodeficiency virus 1 (70413 活性数据)
Human adenovirus 2 (239 活性数据)
Human immunodeficiency virus 2 (5592 活性数据)
L1210 (27553 活性数据)
Vero (26788 活性数据)
MC-38 (857 活性数据)
KBALB cell line (25 活性数据)
L929 (3802 活性数据)
LM (36 活性数据)
rep
Replicase polyprotein 1ab (378 活性数据)
SARS-CoV-2 (38078 活性数据)
作用机制
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
化学和物理性质
溶解性
Soluble in water, methanol, hot ethanol, hot acetone, hot ethyl acetate, pyridine, glacial acetic acid and hydrochloric acid. Slightly soluble in hot chloroform .
密度
1.452
敏感性
对光敏感;易吸潮
比旋光度
33 ° (C=1, 1mol/L NaOH)
熔点
183-191°C
分子量
242.230 g/mol
XLogP3
-1.200
氢键供体数Hydrogen Bond Donor Count
3
氢键受体数Hydrogen Bond Acceptor Count
5
可旋转键计数Rotatable Bond Count
2
精确质量Exact Mass
242.09 Da
单同位素质量Monoisotopic Mass
242.09 Da
拓扑极表面积Topological Polar Surface Area
99.100 Ų
重原子数Heavy Atom Count
17
形式电荷Formal Charge
0
复杂度Complexity
381.000
同位素原子数Isotope Atom Count
0
定义的原子立体中心计数Defined Atom Stereocenter Count
3
未定义的原子立体中心计数Undefined Atom Stereocenter Count
0
定义的键立体中心计数Defined Bond Stereocenter Count
0
未定义的键立体中心计数Undefined Bond Stereocenter Count
0
所有立体化学键的总数The total count of all stereochemical bonds
0
共价键合单元计数Covalently-Bonded Unit Count
1
安全和危险性（GHS）
信号词
危险
危险声明
H302: 吞食有害
H335: 可能引起呼吸道刺激
H340: 可能导致遗传缺陷
H341: 怀疑引起遗传缺陷
预防措施声明
P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾
P264: 处理后要彻底洗手。
P270: 使用本产品时，请勿进食、饮水或吸烟。
P271: 仅在室外或通风良好的地方使用。
P281: 根据需要使用个人防护设备。
P330: 漱口
P304+P340: 如误吸入：将人转移到空气新鲜处，保持呼吸舒适体位。
P405: 密闭存放
P403+P233: 存放在通风良好的地方。保持容器密闭。
P501: 将内容物/容器处理到。。。
P203: 使用前，获取、阅读并遵守所有安全说明。
P301+P317: 如果被吞咽：请寻求医疗帮助。
P318: 如果暴露或担心，请就医。
P319: 如果你感到不适，请寻求医疗帮助。
WGK Germany
3
RTECS
XP2071000
Merck Index
9397
个人防护装备
Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 L2208975 |
分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
| F2602343 |
分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
| C2612640 |
分析证书 | T104771 |
| C2612641 |
分析证书 | T104771 |
| C2612642 |
分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
| D2510431 |
分析证书 | T104771 |
| D2510434 |
分析证书 | T104771 |
| H2527104 |
分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
| G2326671 |
分析证书 | T104771 |
| A2509071 |
分析证书 | T104771 |
| G2326603 |
分析证书 | T104771 |
| G2326597 |
分析证书 | T104771 |
| G2326592 |
分析证书 | T104771 |
| G2326578 |
分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
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分析证书 | T104771 |
| F2201118 |
分析证书 | T104771 |
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分析证书 | T104771 |
| F2201057 |
分析证书 | T104771 |
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此产品的引用文献
引用文献
| 1. Xu Lian, Li Hui-Min, Lin Juan. (2023) Efficient synthesis of 2’-deoxyguanosine in one-pot cascade by employing an engineered purine nucleoside phosphorylase from Brevibacterium acetylicum. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 39 (10): (1-9). [PMID:37606812] [10.1007/s11274-023-03721-1] |
| 2. Fei Wang, Fan Yang, Jiawei Liu, Quan Bai. (2023) Studies on the retention mechanism of solutes in hydrophilic interaction chromatography using stoichiometric displacement theory II. HILIC/RPLC dual-retention mechanism of solutes in hydrophilic interaction chromatography over the entire range of water concentration in mobile phase. TALANTA, [PMID:37385194] [10.1016/j.talanta.2023.124858] |
| 3. Ye Shu, Tong Chen, Tao Liu, Hechen Xu, Jinjin He, Zhihui Cai, Wanning Zhu, Zihan Zhao, Jun Jiang. (2023) Preparation and characterization of mixed-mode hydrophilic stationary phase based on triple hydrophilic functionalized moieties and its application to traditional Chinese herbal compound. MICROCHEMICAL JOURNAL, [10.1016/j.microc.2023.108863] |
| 4. Li Gao, Yuan Wang, Yaqiong Qin, Yaming Sun, Lijun He, Shusheng Zhang, Wenjie Zhao. (2023) Preparation and characterization of glucose-based covalent organic polymer coated silica as stationary phase for high-performance liquid chromatography. JOURNAL OF CHROMATOGRAPHY A, [PMID:36857980] [10.1016/j.chroma.2023.463876] |
| 5. Yan Wu, Kaixing Luo, Yanjuan Liu, Wei Chen, Zhengwu Bai, Sheng Tang. (2022) Innovative preparation of ureido/dodecyl dual-functionalized silica as a versatile mixed-mode stationary phase for high-resolution chromatographic separations. JOURNAL OF CHROMATOGRAPHY A, [PMID:35085896] [10.1016/j.chroma.2022.462834] |
| 6. Jia Ding, Fei Liu, Chunling Qi, Yunlei Zhou, Huanshun Yin, Shiyun Ai. (2021) Enhanced photoactivity of perovskite Bi4NbO8Cl/PTC-NH2 heterojunction and its application for photoelectrochemical sensing of DNA hydroxymethylation. SENSORS AND ACTUATORS B-CHEMICAL, [10.1016/j.snb.2021.130211] |
| 7. Qian Wang, Huanshun Yin, Yunlei Zhou, Jun Wang, Shiyun Ai. (2021) Photoelectrochemical biosensor for 5-formylcytosine deoxyribonucleoside detection based on BiIO4-WS2/CuO ternary heterojunction. SENSORS AND ACTUATORS B-CHEMICAL, [10.1016/j.snb.2021.130019] |
| 8. Tong Chen, Liang Xu, Guangsan Song, Yuan Li, Hong Xu, Hongbin Zhou, Zhen Xiao, Ping Li. (2021) Preparation and application of Au nanoparticles-decorated SO3H-cofunctionalized silica stationary phase for per aqueous liquid chromatography. MICROCHEMICAL JOURNAL, [10.1016/j.microc.2021.105985] |
| 9. Chao Fan, Hao Tang, Licheng Wang, Yijing Li, Xusheng Wang, Shuai Wang, Xiaojing Liang. (2020) The preparation of a core–shell stationary phase by the in situ polymerization of a hydrophilic polymer on the surface of silica and its chromatographic performance. NEW JOURNAL OF CHEMISTRY, 44 (27): (11704-11709). [10.1039/D0NJ01197E] |
| 10. Zhonghua Dong, Yueyue Sun, Guangwei Wei, Siying Li, Zhongxi Zhao. (2019) A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy. MOLECULES, 24 (22): (4119). [PMID:31739543] [10.3390/molecules24224119] |
| 11. Tianpei Cai, Haijuan Zhang, Jia Chen, Zhan Li, Hongdeng Qiu. (2019) Polyethyleneimine-functionalized carbon dots and their precursor co-immobilized on silica for hydrophilic interaction chromatography. JOURNAL OF CHROMATOGRAPHY A, [PMID:30922720] [10.1016/j.chroma.2019.03.026] |
| 12. Tengda Wang, Jianxing Shan, Li Wang, Xiangwen Zhang, Guozhu Li. (2019) On the kinetics of catalytic hydrogenation over Pd nanoparticles regulated by various nucleosides. CHEMICAL ENGINEERING SCIENCE, [10.1016/j.ces.2019.02.014] |
| 13. Cai Tianpei, Zhang Haijuan, Rahman A. F. M. Mustafizur, Shi Yan-Ping, Qiu Hongdeng. (2017) Silica grafted with silanized carbon dots as a nano-on-micro packing material with enhanced hydrophilic selectivity. MICROCHIMICA ACTA, 184 (8): (2629-2636). [10.1007/s00604-017-2277-1] |
| 14. Chaozhan Wang, Huanhuan Xu, Yinmao Wei. (2015) The preparation of high-capacity boronate affinity adsorbents by surface initiated reversible addition fragmentation chain transfer polymerization for the enrichment of ribonucleosides in serum. ANALYTICA CHIMICA ACTA, [PMID:26703260] [10.1016/j.aca.2015.11.013] |
| 15. Qing Wang, Yao Long, Lin Yao, Li Xu, Zhi-Guo Shi, Lanying Xu. (2015) Preparation, characterization and application of a reversed phase liquid chromatography/hydrophilic interaction chromatography mixed-mode C18-DTT stationary phase. TALANTA, [PMID:26695288] [10.1016/j.talanta.2015.09.009] |
| 16. Wang Xue-Jie, You Jin-Zong. (2015) Study on the molecular structure and thermal stability of pyrimidine nucleoside analogs. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY, 120 (1): (1009-1025). [10.1007/s10973-014-4362-6] |
| 17. Yali Yang, Jia Chen, Xiaojing Liang, Bei Liu, Kaijun Quan, Xiuhui Liu, Hongdeng Qiu. (2024) Adjustable chromatographic performance of silica-based mixed-mode stationary phase through the control of co-grafting amounts of imidazole and C18 chain. JOURNAL OF CHROMATOGRAPHY A, [PMID:38598894] [10.1016/j.chroma.2024.464889] |
| 18. Min Zhang, Dandan Guo, Yan Zhu, Shaohua Huang. (2025) Controllable preparation and application of mixed-mode stationary phases based on green peptide coupling reactions. MICROCHEMICAL JOURNAL, [10.1016/j.microc.2025.113347] |
| 19. Xingkang Diao, GuoHua Qi, Xinli Li, Yu Tian, Jing Li, Yongdong Jin. (2025) Label-Free Exosomal SERS Detection Assisted by Machine Learning for Accurately Discriminating Cell Cycle Stages and Revealing the Molecular Mechanisms during the Mitotic Process. ANALYTICAL CHEMISTRY, [PMID:39999424] [10.1021/acs.analchem.4c06240] |
| 20. Jinlei Yang, Jing Wang, Runsong Cheng, Tianyi Liao, Siyuan Pan, Murong Du, Weiliang Liu, Liying Yan, Sichun Zhang, Xinrong Zhang. (2025) Microseparation of Lipophilic and Hydrophilic Metabolites for Single Oocyte Mass Spectrometry Analysis. ANALYTICAL CHEMISTRY, [PMID:39800910] [10.1021/acs.analchem.4c04900] |
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本产品的 CAS 号为 50-89-5，分子式为 C10H14N2O5，分子量为 242.23 g/mol。InChIKey IQFYYKKMVGJFEH-XLPZGREQSA-N。
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