Rabbit anti Human ACTA2 Antibody, Recombinant (RR645), could be used for WB, IHC, Flow, IF, ICC and so on.
Application:
WB: 1/1000 - 1/2000
IHC: 1/2000 - 1/4000
Flow: 1/20 - 1/100
IF/ICC: 1/80 - 1/250
Recombinant ACTA2 Antibody (Ab088446) - Western Blot
All lanes: Recombinant ACTA2 Antibody (Ab088446) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 44 kDa
Exposure time: 80.0 s
Recombinant ACTA2 Antibody (Ab088446) - IF
IF analysis of ACTA2 (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant ACTA2 Antibody (Ab088446) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant ACTA2 Antibody (Ab088446) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta tissue labelling ACTA2 with Recombinant ACTA2 Antibody (Ab088446) at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant ACTA2 Antibody (Ab088446) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ACTA2 Antibody (Ab088446) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant ACTA2 Antibody (Ab088446) (1/100 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Green) was used as a control.
1/1000 - 1/2000
1/2000 - 1/4000
1/20 - 1/100
1/80 - 1/250
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0911322 |
分析证书 | Ab088446 |
| ZJ23F0901100 |
分析证书 | Ab088446 |
| ZJ23F0901101 |
分析证书 | Ab088446 |
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