GAPDH Mouse mAb

功能和特点

规格或纯度: mAb (B08/4A11); Mouse anti Human GAPDH Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
简短描述: Mouse monoclonal (B08/4A11) to GAPDH
反应种属:
亚型: IgG1

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货号 (SKU)	包装规格	是否现货
Ab155825-10μl (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	10μl	现货
Ab155825-50μl	50μL	现货
Ab155825-100μl	100μL	现货
Ab155825-1ml	1ml	期货

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基本信息

产品名称	GAPDH Mouse mAb
别名	重组Anti-GAPDH抗体 \| 小鼠单克隆抗体 to GAPDH
英文别名	38 kDa BFA-dependent ADP-ribosylation substrate antibody \| aging associated gene 9 protein antibody \| Aging-associated gene 9 protein antibody \| BARS-38 antibody \| cb609 antibody \| EC 1.2.1.12 antibody \| Epididymis secretory sperm binding protein Li 162eP
规格或纯度	mAb (B08/4A11); Mouse anti Human GAPDH Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
特异性	GAPDH
免疫原	Recombinant full length human GAPDH
阳性对照	WB: HEK293, HeLa, Jurkat, A431, HepG2 and K562 whole cell lysates; Flow: Jurkat cells; ICC/IF: HeLa cells
偶联标记	1

产品属性

克隆	2
克隆号	B08/4A11
Format	Whole IgG
亚型	IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	PBS, pH 7.2
防腐剂	No
浓度	Lot by Lot
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

应用

Application	Notes
ICC/IF	0.5 μg/mL
WB	1/1000-1/100000 ; Predicted molecular weight: 37 kDa
Flow	0.016 μg/mL- 1 μg/mL

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图片

Western Blot: GAPDH Mouse mAb (Ab155825)

GAPDH Mouse mAb (Ab155825) - Western Blot
All lanes: GAPDH Antibody (Ab155825) at 0.05 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 35.8 kDa
Observed band size: 35.6 kDa
Exposure time: 36.9 s

Flow Cytometry: GAPDH Mouse mAb (Ab155825)

GAPDH Mouse mAb (Ab155825) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with GAPDH Mouse mAb (Ab155825) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.

ICC/IF: GAPDH Mouse mAb (Ab155825)

GAPDH Mouse mAb (Ab155825) - ICC/IF
IF analysis of GAPDH (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Ab155825) at 0.3μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

ICC/IF: GAPDH Mouse mAb (Ab155825)

GAPDH Mouse mAb (Ab155825) - ICC/IF
IF analysis of GAPDH (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with GAPDH Mouse mAb (Ab155825) at 0.3μg/mL and Recombinant COX IV Antibody (Ab097410) (Red) at 1μg/mL in blocking buffer for 2h at room temperature and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 and Goat Anti-Rabbit IgG H&L (Cy5) (Ab170147) for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

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参考文献

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GAPDH Mouse mAb

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