38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 proteinantibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12antibody | Epididymis secretory sperm binding protein Li 162eP a
规格或纯度
ExactAb™, 已验证, 重组, 高性能, 见COA
生化机理
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Post-translational: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. ISGylated.
宿主种属
兔(Rabbit)
特异性
GAPDH
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse),猴(Monkey)
免疫原
A synthetic peptide derived from human GAPDH (AA 1-150).
阳性对照
WB: HeLa, A431, A549, MCF7, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human bladder cancer tissue. IF/ICC: HeLa cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant GAPDH Antibody (Ab104781) - Western Blot All lanes: Recombinant GAPDH Antibody (Ab104781) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: 21.9 s
Recombinant GAPDH Antibody (Ab104781) - IHC Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Recombinant GAPDH Antibody (Ab104781).
Recombinant GAPDH Antibody (Ab104781) - IF/ICC Immunofluorescent analysis of HeLa using the Recombinant GAPDH Antibody (Ab104781) at 1/50 dilution.
Recombinant GAPDH Antibody (Ab104781) - IF/ICC Immunofluorescent analysis of HeLa using the Recombinant GAPDH Antibody (Ab104781) at 1/50 dilution.
Recombinant GAPDH Antibody (Ab104781) - IF/ICC Immunofluorescent analysis of Hela cells, using Recombinant GAPDH Antibody (Ab104781).
Recombinant GAPDH Antibody (Ab104781) - Western Blot
All lanes: Recombinant GAPDH Antibody (Ab104781) at 1/100000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
ZJ26F0131019
危险品化学品经营许可证（带存储）