ExactAb™,已验证,重组,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human GAPDH Antibody, Recombinant (RR688), could be used for WB, IHC, Flow, IF, ICC, IP and so on.
Application:
WB: 1/5000 - 1/40000
IHC: 1/1600 - 1/3200
Flow: 1/200 - 1/1000
IF/ICC: 1/2000 - 1/10000
IP: 1/50
Protein Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Post-translational
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated.
Recombinant GAPDH Antibody (Ab104788) - Western Blot
All lanes: Recombinant GAPDH Antibody (Ab104788) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 1.0 s
Recombinant GAPDH Antibody (Ab104788) - IF
IF analysis of GAPDH (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant GAPDH Antibody (Ab104788) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant GAPDH Antibody (Ab104788) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of prostate cancer tissue labelling GAPDH with Recombinant GAPDH Antibody (Ab104788) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant GAPDH Antibody (Ab104788) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant GAPDH Antibody (Ab104788) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant GAPDH Antibody (Ab104788) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant GAPDH Antibody (Ab104788) - IP
GAPDH was immunoprecipitated from 0.1 mg of HeLa whole cell lysate with Recombinant GAPDH Antibody (Ab104788) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant GAPDH Antibody (Ab104788) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant GAPDH Antibody (Ab104788) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 2 μg (input).
1/50
1/1600-1/3200
1/200 - 1/1000
1/5000 - 1/40000
1/2000 - 1/10000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131028 |
分析证书 | Ab104788 |
| ZJ26F0131027 |
分析证书 | Ab104788 |
| ZJ26F0131026 |
分析证书 | Ab104788 |
| ZJ26F0131025 |
分析证书 | Ab104788 |
| ZJ23F0901096 |
分析证书 | Ab104788 |
| ZJ23F0901097 |
分析证书 | Ab104788 |
| ZJ24F1216244 |
分析证书 | Ab104788 |
| ZJ24F0505915 |
分析证书 | Ab104788 |
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