计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 3195 篇同行评审文献引用。
氨水又称氢氧化氨,分子量为35.05,分子式为NH₄OH,CAS号为1336-21-6 ,氨水是氨气的水溶液,无色透明且具有刺激性气味。HE染色或其他组织染色后的返蓝很重要,苏木素在酸性条件下处于红色离子状态,呈红色;在碱性条件下处于蓝色离子状态,呈蓝色;组织切片经盐酸乙醇分化后呈红色或粉红色,故分化之后,立即用水除去组织切片上的酸而中止分化,再用弱碱性水使苏木素染上的细胞核呈现蓝色,这个过程称为返蓝作用或蓝化作用。
氨水溶液 (0.1%)主要由氨水、去离子水组成,经常用于HE染色或其他组织染色后的返蓝,是一种非常重要的辅助试剂。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、根据实验具体要求操作,蓝化后应立即用水冲洗干净。
2、一般蓝化3-30s,应根据具体组织和切片厚度调整时间。
注意事项:
1、密闭保存,一旦开启尽快用完,因为其有效成分易挥发。
2、试剂开封后请尽快使用,以防影响后续实验效果。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Ammonia water, also known as ammonium hydroxide, has a molecular weight of 35.05, a molecular formula of NH₄OH, and a CAS number of 1336-21-6. It is an aqueous solution of ammonia, which is colorless, transparent, and has a pungent odor. The blueing process after HE staining (Hematoxylin-Eosin staining) or other tissue staining is of great importance. Hematoxylin exists in a red ionic state under acidic conditions, showing a red color; under alkaline conditions, it is in a blue ionic state, presenting a blue color. After differentiation with hydrochloric acid-ethanol, tissue sections turn red or pink. Therefore, immediately after differentiation, water should be used to remove the acid from the tissue sections to terminate the differentiation process, and then weakly alkaline water is applied to make the cell nuclei stained by hematoxylin appear blue. This process is called the blueing effect or bluing process.
Ammonia water solution (0.1%) is mainly composed of ammonia water and deionized water. It is often used for the blueing process after HE staining or other tissue staining, and is a very important auxiliary reagent. This reagent is only for scientific research purposes and is not suitable for clinical diagnosis or other applications.
Operating Procedures (for reference only)
1. Operate in accordance with the specific requirements of the experiment. After bluing, the sections should be rinsed thoroughly with water immediately.
2. Generally, the bluing time ranges from 3 to 30 seconds, and the duration should be adjusted based on the specific type of tissue and the thickness of the sections.
Precautions
1. Store in a sealed container. Once opened, use it as soon as possible, as its active ingredients are volatile.
2. After opening the reagent, use it promptly to avoid affecting the results of subsequent experiments.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | A1507829 |
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