氨水溶液 (0.1%)

CAS: 1336-21-6 货号: A1507829 分子式: H5NO 分子量: 35.05 Beilstein号: 3587154 EC号: 215-647-6
有货
级别和纯度: BioReagent ? 生物试剂级(BioReagent)—— 经测试适用于生命科学和分子生物学。适用于需要生物相容性的细胞培养、检测和生化工作。 生物染色剂 ? 生物染色剂级 —— 经表征用于细胞和组织染色的染料。适用于注重染色一致性的组织学和显微镜检查。 用于显微镜 ? 显微镜级 —— 适用于样品制备和成像的试剂/染料。适用于需要清晰度和低背景的显微镜检查。 0.1%
别名
氢氧化铵溶液 (0.1%); | 氢氧化铵溶液 (0.1%); | 氢氧化铵溶液
储存条件
避光,室温
运输条件
常规运输
应用
细胞染色
★
规格
库存
价格
数量
500ml
A1507829-500ml
现货 Stock Image
¥99.90
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为什么选择此级别

BioReagent,生物染色剂,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

避光,室温。常规运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 3195 篇同行评审文献引用。

概述

  氨水又称氢氧化氨,分子量为35.05,分子式为NH₄OH,CAS号为1336-21-6 ,氨水是氨气的水溶液,无色透明且具有刺激性气味。HE染色或其他组织染色后的返蓝很重要,苏木素在酸性条件下处于红色离子状态,呈红色;在碱性条件下处于蓝色离子状态,呈蓝色;组织切片经盐酸乙醇分化后呈红色或粉红色,故分化之后,立即用水除去组织切片上的酸而中止分化,再用弱碱性水使苏木素染上的细胞核呈现蓝色,这个过程称为返蓝作用或蓝化作用。

  氨水溶液 (0.1%)主要由氨水、去离子水组成,经常用于HE染色或其他组织染色后的返蓝,是一种非常重要的辅助试剂。该试剂仅用于科研领域,不适用于临床诊断或其他用途。

操作步骤(仅供参考):

1、根据实验具体要求操作,蓝化后应立即用水冲洗干净。

2、一般蓝化3-30s,应根据具体组织和切片厚度调整时间。

注意事项:

1、密闭保存,一旦开启尽快用完,因为其有效成分易挥发。

2、试剂开封后请尽快使用,以防影响后续实验效果。

3、为了您的安全和健康,请穿实验服并戴一次性手套操作。

  Ammonia water, also known as ammonium hydroxide, has a molecular weight of 35.05, a molecular formula of NH₄OH, and a CAS number of 1336-21-6. It is an aqueous solution of ammonia, which is colorless, transparent, and has a pungent odor. The blueing process after HE staining (Hematoxylin-Eosin staining) or other tissue staining is of great importance. Hematoxylin exists in a red ionic state under acidic conditions, showing a red color; under alkaline conditions, it is in a blue ionic state, presenting a blue color. After differentiation with hydrochloric acid-ethanol, tissue sections turn red or pink. Therefore, immediately after differentiation, water should be used to remove the acid from the tissue sections to terminate the differentiation process, and then weakly alkaline water is applied to make the cell nuclei stained by hematoxylin appear blue. This process is called the blueing effect or bluing process.

  Ammonia water solution (0.1%) is mainly composed of ammonia water and deionized water. It is often used for the blueing process after HE staining or other tissue staining, and is a very important auxiliary reagent. This reagent is only for scientific research purposes and is not suitable for clinical diagnosis or other applications.

Operating Procedures (for reference only)

1. Operate in accordance with the specific requirements of the experiment. After bluing, the sections should be rinsed thoroughly with water immediately.

2. Generally, the bluing time ranges from 3 to 30 seconds, and the duration should be adjusted based on the specific type of tissue and the thickness of the sections.

Precautions

1. Store in a sealed container. Once opened, use it as soon as possible, as its active ingredients are volatile.

2. After opening the reagent, use it promptly to avoid affecting the results of subsequent experiments.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

规格

别名
氢氧化铵溶液 (0.1%); | 氢氧化铵溶液 (0.1%); | 氢氧化铵溶液
英文别名
Ammonia Solution (0.1%)
规格或纯度
BioReagent,生物染色剂,用于显微镜,0.1%
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Ammonia Solution (0.1%)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
EC号
215-647-6
分子类型
生物试剂/缓冲液
Isomeric SMILES
[NH4+].[OH-]
UN Number
Packing Group
III
分子量
35.05
Beilstein号
3587154
Reaxy-Rn
11342939
Reaxys-RN link address
https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=11342939&ln=

技术文档

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高级数据

化学和物理性质
敏感性
Light-sensitive
闪点(℃)
132°C
沸点
-33°C
熔点
-69°C(lit.)
安全和危险性(GHS)
象形图
GHS05,   GHS09
信号词
危险
危险声明

H314: 造成严重的皮肤灼伤和眼睛损伤

H400: 对水生生物有剧毒

Class
8
个人防护装备
Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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批号(Lot Number) 证书类型 货号
ZJ26F0939250 分析证书 A1507829
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此产品的引用文献
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