Recombinant AKT1/AKT2/AKT3 Antibody

货号: Ab087925
有货
级别和纯度: 重组 ? 重组(Recombinant)—— 通过重组表达生产,序列确定、批次稳定。适用于来源明确、无动物源的可重现蛋白。 ExactAb™ ? ExactAb™ —— 阿拉丁强调精准和验证质量的抗体系列。适用于可靠、特异性经验证的抗体应用。 已验证 ? 已验证级 —— 发布前在特定应用中确认性能。当需要试剂按声称工作的书面保证时使用。 见COA
应用
IF/ICC, IHC, WB
宿主种属
兔(Rabbit)
种属反应性
人(Human), 仓鼠(Hamster), 大鼠(Rat), 小鼠(Mouse)
亚型
Rabbit IgG
偶联
Unconjugated
★
规格
库存
价格
数量
10μl
Ab087925-10μl
现货 Stock Image
¥374.90
50μL
Ab087925-50μl
现货 Stock Image
¥974.90
100μL
Ab087925-100μl
现货 Stock Image
¥1,574.90
1ml
Ab087925-1ml
期货 Stock Image
¥16,999.90
请输入您要添加的产品数量
🧪
为什么选择此级别

ExactAb™,已验证,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

📚

文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Rabbit anti Human AKT1/AKT2/AKT3 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

规格

产品名称
Recombinant AKT1/AKT2/AKT3 Antibody
别名
AKT1/AKT2/AKT3抗体 | 蛋白激酶AKT1/AKT2/AKT3抗体 | 重组 AKT1/AKT2/AKT3 抗体 | 蛋白激酶AKT1/2/3抗体
英文别名
AKT antibody | AKT1 antibody | AKT1 kinase antibody | AKT1m antibody | AKT2 antibody | AKT2 kinase antibody | Akt3 antibody | AKT3_HUMAN antibody | CAKT antibody | CWS6 antibody | DKFZp434N0250 antibody | HIHGHH antibody | kinase Akt1 antibody | MGC99656
规格或纯度
重组,ExactAb™,已验证,见COA
生化机理
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
宿主种属
兔(Rabbit)
特异性
AKT1/AKT2/AKT3
种属反应性
人(Human),仓鼠(Hamster),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human AKT1/2/3 (AA 281-480).
阳性对照
WB: HeLa, Jurkat, NIH/3T3, A549 and MCF7 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
亚型
IgG
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂
0.01% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃(1-2周);长期储存-20℃(24个月);收货后建议分装,避免反复冻融。
图片

Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - Western Blot
All lanes: Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 57 kDa
Exposure time: 2.7 s

Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - IF/ICC 
IF analysis of Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - IF/ICC 
The HeLa cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubated with Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) (1/100) at room temperature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.
应用
应用名称
稀释比例
WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/100

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

关联靶点(人)
AKT3 Tchem RAC-γ 丝氨酸/苏氨酸蛋白激酶(RAC-gamma serine/threonine-protein kinase) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
AKT2 Tchem RAC-β丝氨酸/苏氨酸蛋白激酶(RAC-beta serine/threonine-protein kinase) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
AKT1 Tchem RAC-alpha 丝氨酸/苏氨酸蛋白激酶(RAC-alpha serine/threonine-protein kinase) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
AKT2 Tchem Serine/threonine-protein kinase AKT2 (4301 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
AKT3 Tchem Serine/threonine-protein kinase AKT3 (3157 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
AKT1 Tchem Serine/threonine-protein kinase AKT (9192 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
AKT2 Tchem Serine/threonine-protein kinase AKT (245 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
AKT2 Tchem Serine/threonine-protein kinase Rac alpha/beta (1 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
AKT1 Tchem VHL/AKT1 (26 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
AKT1 Tchem CRBN/AKT1 (26 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
AKT2 Tchem CRBN/AKT2 (2 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
作用机制
作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到8个结果

批号(Lot Number) 证书类型 货号
ZJ24F1011769 分析证书 Ab087925
ZJ25F1129806 分析证书 Ab087925
ZJ25F1129807 分析证书 Ab087925
ZJ25F1129808 分析证书 Ab087925
ZJ23F0901114 分析证书 Ab087925
ZJ23F0901115 分析证书 Ab087925
ZJ25F0319604 分析证书 Ab087925
ZJ25F0319510 分析证书 Ab087925
技术文档和文章
组织块整体免疫组织化学染色 实验方案和技巧
Protocol and tips for whole mount immunohistochemical staining
冷冻样本的免疫组织化学染色实验方案(IHC-Fr)
免疫组织化学染色的抗原修复方案
AKT通路的组成、激活机制与生物学效应
Composition, Activation Mechanisms, and Biological Effects of the AKT Pathway
Angiopoietin-TIE2信号通路的血管稳态、通透性调控与病理性血管重塑机制
Angiopoietin-TIE2 Signaling Pathway: Mechanisms of Vascular Homeostasis, Permeability Regulation, and Pathological Vascular Remodeling
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Immunohistochemistry (IHC) staining antigen retrieval protocol
βc家族细胞因子的共享受体机制及其在造血与免疫调控中的作用
Shared Receptor Mechanisms of βc Family Cytokines and Their Roles in Hematopoietic and Immune Regulation
IGFBP家族在IGF信号调控及疾病生物标志物研究中的作用
Role of the IGFBP Family in IGF Signaling Regulation and Disease Biomarker Research
溶液计算器

需要帮助选择级别?

我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。

查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 →