ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 2 篇同行评审文献引用。
Application:
WB: 1/500 - 1/2000
IF/ICC: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000
Protein Function
Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
AKT1 Mouse mAb (Ab087937) - Western Blot
All lanes: AKT1 Mouse mAb (Ab087937) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 55.7 kDa
Observed band size: 55.1 kDa
AKT1 Mouse mAb (Ab087937) - Flow Cytometry
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling AKT1 with AKT1 Mouse mAb (Ab087937) at 1/200 dilution (green) compared with a negative control (red).
AKT1 Mouse mAb (Ab087937) - ICC/IF
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AKT1 (green) using AKT1 Mouse mAb (Ab087937) in ICC/IF. Blue: DRAQ5 DNA dye. Red: Actin filaments stained with AF-555-Phalloidin.
1/500-1/2000
1/200-1/1000
1/200-1/400
1/10000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ23F0700655 |
分析证书 | Ab087937 |
| ZJ23F0700656 |
分析证书 | Ab087937 |
| ZJ24F1011903 |
分析证书 | Ab087937 |
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