Rabbit anti Human AMPK beta 1 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Recombinant AMPK beta 1 Antibody (Ab088673) - Western Blot All lanes: Recombinant AMPK beta 1 Antibody (Ab088673) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 31, 35 kDa The expression profile and molecular weight observed may be due to phosphorylation. Exposure time: 23.6 s
Recombinant AMPK beta 1 Antibody (Ab088673) - Western Blot All lanes: Recombinant AMPK beta 1 Antibody (Ab088673) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 31, 35 kDa The expression profile and molecular weight observed may be due to phosphorylation. Exposure time: 100.0 s