Rabbit anti Human Angiotensin Converting Enzyme 1 Antibody, Recombinant, could be used for WB and so on. Application:
WB: 1/500-1/1000
IF/ICC: 1/200
Protein Function: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
Recombinant Angiotensin Converting Enzyme 1 Antibody (Ab088828) - Western Blot All lanes: Recombinant Angiotensin Converting Enzyme 1 Antibody (Ab088828) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 150 kDa Observed band size: 181 kDa Exposure time: 25.9 s
Recombinant Angiotensin Converting Enzyme 1 Antibody (Ab088828) - IF/ICC IF analysis of Recombinant Angiotensin Converting Enzyme 1 Antibody (Ab088828) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Angiotensin Converting Enzyme 1 Antibody (Ab088828) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).