ExactAb™,已验证,重组,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Bax Antibody, Recombinant (RR647), could be used for WB, IHC, Flow, IP and so on.
Application:
WB: 1/1000 – 1/2500
IHC: 1/1600-1/3200
Flow: 1/100-1/400
IP: 1/25
IF/ICC:1/1000
Protein Function
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Recombinant Bax Antibody (Ab090652) - Western Blot
All lanes: Recombinant Bax Antibody (Ab090652) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 20 kDa
Exposure time: 6.2 s
Recombinant Bax Antibody (Ab090652) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling Bax with Recombinant Bax Antibody (Ab090652) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant Bax Antibody (Ab090652) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant Bax Antibody (Ab090652) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant Bax Antibody (Ab090652) (1/400 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant Bax Antibody (Ab090652) - IP
Bax was immunoprecipitated from 0.5 mg HT-29 whole cell lysate with Recombinant Bax Antibody (Ab090652) at 1/25 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant Bax Antibody (Ab090652) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Bax Antibody (Ab090652) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 30 s.
Recombinant Bax Antibody (Ab090652) - IF/ICC
IF analysis of Recombinant Bax Antibody (Ab090652) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Bax Antibody (Ab090652) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab170148) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/25
1/1600-1/3200
1/1000-1/2500
1/100-1/400
1:1000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0404255 |
分析证书 | Ab090652 |
| ZJ23F0901011 |
分析证书 | Ab090652 |
| ZJ23F0901012 |
分析证书 | Ab090652 |
| ZJ25F0319505 |
分析证书 | Ab090652 |
| ZJ24F0911347 |
分析证书 | Ab090652 |
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