Recombinant CD90/Thy1 Antibody (Ab095500) - Western Blot All lanes: Recombinant CD90/Thy1 Antibody (Ab095500) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 18 kDa Observed band size: 27 kDa Exposure time: 3.0 s
Recombinant CD90/Thy1 Antibody (Ab095500) - IHC Immunohistochemical analysis of paraffin-embedded human tonsil, using Recombinant CD90/Thy1 Antibody (Ab095500) .
Recombinant CD90/Thy1 Antibody (Ab095500) - IF/ICC IF analysis of Recombinant CD90/Thy1 Antibody (Ab095500) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant CD90/Thy1 Antibody (Ab095500) at 1/300 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).