ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Mouse Monoclonal (2G1D5) to CHK1, could be used for WB and ELISA.
Application:
WB: 1/500 - 1/2000
ELISA: 1/10000
Protein Function:
CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Chk1 Mouse mAb (Ab096310) - Western Blot
All lanes: Chk1 Mouse mAb (Ab096310) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Chk1 Mouse mAb (Ab096310) - Western Blot
All lanes: Chk1 Mouse mAb (Ab096310) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
1/500-1/2000
1/10000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ23F0700740 |
分析证书 | Ab096310 |
| ZJ23F0700741 |
分析证书 | Ab096310 |
| ZJ26F0737181 |
分析证书 | Ab096310 |
| ZJ24F0303042 |
分析证书 | Ab096310 |
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