Rabbit anti Human Cullin 2/CUL-2 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/1000-1/5000 Protein Function: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Recombinant Cullin 2/CUL-2 Antibody (Ab098172) - Western Blot All lanes: Recombinant Cullin 2/CUL-2 Antibody (Ab098172) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 87 kDa Observed band size: 87, 91 kDa Exposure time: 20.2 s