EGFR Mouse mAb

货号: Ab101163
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 ExactAb™ ? ExactAb™ —— 阿拉丁强调精准和验证质量的抗体系列。适用于可靠、特异性经验证的抗体应用。 已验证 ? 已验证级 —— 发布前在特定应用中确认性能。当需要试剂按声称工作的书面保证时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 见COA
应用
IF/ICC, IHC, WB
宿主种属
小鼠(Mouse)
种属反应性
人(Human), 小鼠(Mouse)
亚型
Mouse IgG2b
克隆号
C4
偶联
Unconjugated
★
规格
库存
价格
数量
10μl
Ab101163-10μl
现货 Stock Image
¥374.90
50μL
Ab101163-50μl
现货 Stock Image
¥974.90
100μL
Ab101163-100μl
现货 Stock Image
¥1,574.90
1ml
Ab101163-1ml
期货 Stock Image
¥13,999.90
请输入您要添加的产品数量
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为什么选择此级别

ExactAb™,已验证,无载体,高性能 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Mouse anti Human EGFR Antibody, Monoclonal(C4), could be used for WB, IHC and so on.

规格

产品名称
EGFR Mouse mAb
别名
表皮生长因子受体抗体 | 表皮生长因子受体单克隆抗体 | EGFR抗体 | EGFR单克隆抗体
英文别名
ErbB-1 | ErbB1 | ER1 | ERB-B1 | MENA | HER1 | Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian | Proto-oncogene c-ErbB-1 | Receptor tyrosine-protein kinase erbB-1 | Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody | Cell
规格或纯度
无载体, ExactAb™, 已验证, 高性能, 见COA
生化机理
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.
宿主种属
小鼠(Mouse)
特异性
EGFR
种属反应性
人(Human),小鼠(Mouse)
免疫原
Recombinant EGFR expressed in E.coli (AA 888-1210)
阳性对照
WB: A431, HeLa, MCF7, A549, NIH/3T3 and HUVEC cell lysates. IHC: Human kidney, placent, lung cancer, stomach, adrenal gland, liver cancer tissues. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
C4
Format
Whole IgG
亚型
IgG2b
轻链亚型
kappa
纯化方法
Protein A + Protein G purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4
防腐剂
0.02% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
图片

EGFR Mouse mAb (Ab101163) - Western Blot
All lanes: EGFR Mouse mAb (Ab101163) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 175 kDa
Observed band size: 169 & 69 kDa
Exposure time: 10.4 s

EGFR Mouse mAb (Ab101163) - Western Blot
All lanes: EGFR Mouse mAb (Ab101163) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 175 kDa
Observed band size: 169 & 69 kDa
Exposure time: 2.3 s

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Lung cancer Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Stomach Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Adrenal gland Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IHC
DAB staining on IHC
Sample: Human Liver cancer Tissue
Primary Ab: 5 μg/mL EGFR Mouse mAb (Ab101163)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

EGFR Mouse mAb (Ab101163) - IF/ICC
IF analysis of EGFR Mouse mAb (Ab101163) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with EGFR Mouse mAb (Ab101163) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

应用
应用名称
稀释比例
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

IF/ICC

5 µg/mL

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

关联靶点(人)
EGFR Tclin 表皮生长因子受体(Epidermal growth factor receptor) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
EGFR Tclin Epidermal growth factor receptor erbB1 (33727 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
EGFR Tclin MER intracellular domain/EGFR extracellular domain chimera (2 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
EGFR Tclin Protein cereblon/Epidermal growth factor receptor (191 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
EGFR Tclin EGFR/PPP1CA (1 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
EGFR Tclin VHL/EGFR (323 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
作用机制
作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到7个结果

批号(Lot Number) 证书类型 货号
ZJ24F0404291 分析证书 Ab101163
ZJ23F1001208 分析证书 Ab101163
ZJ23F1001209 分析证书 Ab101163
ZJ25F0420448 分析证书 Ab101163
ZJ25F0420447 分析证书 Ab101163
ZJ25F0420444 分析证书 Ab101163
ZJ25F0319470 分析证书 Ab101163
技术文档和文章
石蜡样品的免疫组化染色方案(IHC-P)
冷冻样本的免疫组织化学染色实验方案(IHC-Fr)
免疫组织化学染色的抗原修复方案
Immunohistochemistry staining with samples in paraffin (IHC-P)
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Immunohistochemistry (IHC) staining antigen retrieval protocol
HER3信号网络在肿瘤进展、治疗耐药与靶向干预中的作用
Role of the HER3 Signaling Network in Tumor Progression, Therapeutic Resistance, and Targeted Intervention
溶液计算器

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