Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
宿主种属
兔(Rabbit)
特异性
ACSL4
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human FACL4 (AA 661-674)
阳性对照
WB: K562, HeLa, Hep G2, HEK293, MDA-MB-231, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Recombinant FACL4 Antibody (Ab102626) - Western Blot All lanes: Recombinant FACL4 Antibody (Ab102626) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 1.6 s
Recombinant FACL4 Antibody (Ab102626) - Western Blot All lanes: Recombinant FACL4 Antibody (Ab102626) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 79 kDa Observed band size: 76 kDa Exposure time: 24.4 s
Recombinant FACL4 Antibody (Ab102626) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant FACL4 Antibody (Ab102626). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant FACL4 Antibody (Ab102626) - IF/ICC IF analysis of Recombinant FACL4 Antibody (Ab102626) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant FACL4 Antibody (Ab102626) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).