Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
宿主种属
兔(Rabbit)
特异性
Glucose Transporter GLUT1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant GLUT1 expressed in E.coli (AA 251-329)
阳性对照
WB: A549, HeLa, Jurkat, HT-29 and NIH/3T3 cell lysates. IF/ICC: HeLa cells.
Glucose Transporter GLUT1 Antibody (Ab105588) - Western Blot All lanes: Glucose Transporter GLUT1 Antibody (Ab105588) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa Observed band size: 50 - 75 kDa Exposure time: 14.1 s
Glucose Transporter GLUT1 Antibody (Ab105588) - IF/ICC IF analysis of Glucose Transporter GLUT1 Antibody (Ab105588) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Glucose Transporter GLUT1 Antibody (Ab105588) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).