Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
宿主种属
兔(Rabbit)
特异性
HDAC3
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human HDAC3 (AA 400-428).
阳性对照
WB: HeLa, MCF7, K562, Jurkat, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells. IP: HeLa cells.
Recombinant HDAC3 Antibody (Ab107042) - Western Blot All lanes: Recombinant HDAC3 Antibody (Ab107042) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa Observed band size: 47 kDa Exposure time: 5.0 s
Recombinant HDAC3 Antibody (Ab107042) - Western Blot All lanes use Recombinant HDAC3 Antibody (Ab107042) at 1/1000 dilution for 1 hour at room temperature.
Recombinant HDAC3 Antibody (Ab107042) - IP Immunoprecipitate (IP) analysis using Recombinant HDAC3 Antibody (Ab107042) at 1/50 dilution. (WB with Recombinant HDAC3 Antibody (Ab107042) at 1/3000 dilution)
Recombinant HDAC3 Antibody (Ab107042) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant HDAC3 Antibody (Ab107042) at 1/50 dilution.
Recombinant HDAC3 Antibody (Ab107042) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant HDAC3 Antibody (Ab107042) at 1/50 dilution.
ZJ24F0303301
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