Rabbit anti Human LIM Kinase 1 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Post-translational Autophosphorylated. Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1. Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
Recombinant LIM Kinase 1 Antibody (Ab113138) - Western Blot All lanes: Recombinant LIM Kinase 1 Antibody (Ab113138) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 73 kDa Observed band size: 76 kDa Exposure time: 94.3 s
Recombinant LIM Kinase 1 Antibody (Ab113138) - Western Blot All lanes: Recombinant LIM Kinase 1 Antibody (Ab113138) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 73 kDa Observed band size: 85 kDa Exposure time: 94.3 s