Rabbit anti Human LINE-1 ORF1p Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500 - 1/1000 Relevance Domain: The coiled coil domain mediates homotrimerization. The RRM and the CTD domain are both required for proper RNA-binding activity. Function: Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded. Long interspersed element-1/LINE-1/L1 retrotransposons are present in more than 500'000 full (6 kb) or truncated copies in the human genome. Most of them are inactive but 80 to 100 of those elements could be transcribed, translated and active in any individual. An active LINE-1 encodes for 2 proteins translated from a single RNA containing 2 non-overlapping ORFs, ORF1 and ORF2. ORF1p is described in this entry as a representative of all ORF1p potentially expressed by active elements. ORF2p is described in the related entry AC O00370. PTM: Polyubiquitinated, probably by UBR2, which induces its degradation. Similarity: Belongs to the transposase 22 family.
Recombinant LINE-1 ORF1p Antibody (Ab113189) - Western Blot All lanes: Recombinant LINE-1 ORF1p Antibody (Ab113189) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 39 kDa Exposure time: 56.9 s
Recombinant LINE-1 ORF1p Antibody (Ab113189) - Western Blot All lanes: Recombinant LINE-1 ORF1p Antibody (Ab113189) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 10.0 s